Short-term binding and hydrolysis of cyclic 3':5'-adenosine monophosphate by 
aggregating Dictyostelium cells

Malchow, D; Gerisch, G

doi:10.1073/pnas.71.6.2423

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Short-term binding and hydrolysis of cyclic 3':5'-adenosine monophosphate by aggregating Dictyostelium cells

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Malchow, D
Gerisch Group, Friedrich Miescher Laboratory, Max Planck Society;

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Gerisch, G
Gerisch Group, Friedrich Miescher Laboratory, Max Planck Society;

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Citation

Malchow, D., & Gerisch, G. (1974). Short-term binding and hydrolysis of cyclic 3':5'-adenosine monophosphate by aggregating Dictyostelium cells. Proceedings of the National Academy of Sciences of the United States of America, 71(6), 2423-2427. doi:10.1073/pnas.71.6.2423.

Cite as: https://hdl.handle.net/21.11116/0000-000C-808D-4

Abstract

Transient binding of cyclic AMP to aggregating cells of Dictyostelium discoideum was measured under cyclic GMP excess in order to inhibit cyclic AMP hydrolysis by cell-bound phosphodiesterase. Cyclic GMP extended the period of half-maximal cyclic AMP binding from less than 5 sec to 1-2 min. With the same time course as bound cyclic AMP was released from the cells, labeled 5'-AMP appeared in the medium. Specificity, kinetics, and developmental regulation suggest that the cyclic AMP-binding sites exposed in living cells are identical with receptor sites for the chemotactic response. The functioning of the cyclic AMP-receptor/phosphodiesterase system and its formal similarity with the synaptic acetylcholine-receptor/esterase system are discussed.