Artificial cytoskeleton assembly for synthetic cell motility

Sauter, Désirée; Schröter, Martin; Frey, Christoph; Weber, Cornelia; Mersdorf, Ulli; Janiesch, Jan-Willi; Platzman, Ilia; Spatz, Joachim P.

doi:10.1002/mabi.202200437

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Artificial cytoskeleton assembly for synthetic cell motility

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Sauter, Désirée
Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society;

/persons/resource/persons224532

Schröter, Martin
Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society;

/persons/resource/persons201484

Frey, Christoph
Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society;

/persons/resource/persons249566

Weber, Cornelia
Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society;

/persons/resource/persons118670

Mersdorf, Ulli
Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society;

/persons/resource/persons75624

Janiesch, Jan-Willi
Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society;

/persons/resource/persons84351

Platzman, Ilia
Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society;

/persons/resource/persons76135

Spatz, Joachim P.
Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society;
Max Planck School Matter to Life, Max Planck Schools, Max Planck Society;

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引用

Sauter, D., Schröter, M., Frey, C., Weber, C., Mersdorf, U., Janiesch, J.-W., Platzman, I., & Spatz, J. P. (2023). Artificial cytoskeleton assembly for synthetic cell motility. Macromolecular Bioscience, 23(8):, pp. 1-10. doi:10.1002/mabi.202200437.

引用: https://hdl.handle.net/21.11116/0000-000C-81B2-8

要旨

Imitation of cellular processes in cell-like compartments is a current research focus in synthetic biology. Here, a method is introduced for assembling an artificial cytoskeleton in a synthetic cell model system based on a poly(N-isopropyl acrylamide) (PNIPAM) composite material. Toward this end, a PNIPAM-based composite material inside water-in-oil droplets that are stabilized with PNIPAM-functionalized and commercial fluorosurfactants is introduced. The temperature-mediated contraction/release behavior of the PNIPAM-based cytoskeleton is investigated. The reversibility of the PNIPAM transition is further examined in bulk and in droplets and it could be shown that hydrogel induced deformation could be used to controllably manipulate droplet-based synthetic cell motility upon temperature changes. It is envisioned that a combination of the presented artificial cytoskeleton with naturally occurring components might expand the bandwidth of the bottom-up synthetic biology.