English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
Add to Basket
  DetailsSummary

Released
Journal Article

Unraveling the kinetochore nanostructure in Schizosaccharomyces pombe using multi-color SMLM imaging

MPS-Authors

Turkowyd,  Bartosz
Max Planck Institute for Terrestrial Microbiology, Max Planck Society;
Department of Systems and Synthetic Microbiology, Max Planck Institute for Terrestrial Microbiology, Max Planck Society;

/persons/resource/persons256432

Endesfelder,  Ulrike
Max Planck Institute for Terrestrial Microbiology, Max Planck Society;
Department of Systems and Synthetic Microbiology, Max Planck Institute for Terrestrial Microbiology, Max Planck Society;

External Resource

https://doi.org/10.1083/jcb.202209096
(Publisher version)

Fulltext (restricted access)
There are currently no full texts shared for your IP range.
Fulltext (public)
There are no public fulltexts stored in PuRe
Supplementary Material (public)
There is no public supplementary material available
Citation

Virant, D., Vojnovic, I., Winkelmeier, J., Endesfelder, M., Turkowyd, B., Lando, D., et al. (2023). Unraveling the kinetochore nanostructure in Schizosaccharomyces pombe using multi-color SMLM imaging. The Journal of cell biology, 222(4): e202209096. doi:10.1083/jcb.202209096.


Cite as: https://hdl.handle.net/21.11116/0000-000C-91A4-6
Abstract
The key to ensuring proper chromosome segregation during mitosis is the kinetochore (KT), a tightly regulated multiprotein complex that links the centromeric chromatin to the spindle microtubules and as such leads the segregation process. Understanding its architecture, function, and regulation is therefore essential. However, due to its complexity and dynamics, only its individual subcomplexes could be studied in structural detail so far. In this study, we construct a nanometer-precise in situ map of the human-like regional KT of Schizosaccharomyces pombe using multi-color single-molecule localization microscopy. We measure each protein of interest (POI) in conjunction with two references, cnp1CENP-A at the centromere and sad1 at the spindle pole. This allows us to determine cell cycle and mitotic plane, and to visualize individual centromere regions separately. We determine protein distances within the complex using Bayesian inference, establish the stoichiometry of each POI and, consequently, build an in situ KT model with unprecedented precision, providing new insights into the architecture. © 2023 Virant et al.