Structural Organization of Unique Retrovirus-like Particles Budding from Human 
Teratocarcinoma Cell Lines

Boller, K; Frank, H; Löwer, J; Löwer, R; Kurth, R

doi:10.1099/0022-1317-64-12-2549

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Structural Organization of Unique Retrovirus-like Particles Budding from Human Teratocarcinoma Cell Lines

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Löwer, R
Kurth Group, Friedrich Miescher Laboratory, Max Planck Society;

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引用

Boller, K., Frank, H., Löwer, J., Löwer, R., & Kurth, R. (1983). Structural Organization of Unique Retrovirus-like Particles Budding from Human Teratocarcinoma Cell Lines. Journal of General Virology, 64(12), 2549-2559. doi:10.1099/0022-1317-64-12-2549.

引用: https://hdl.handle.net/21.11116/0000-000C-90B1-8

要旨

Human teratocarcinoma cells cultured in vitro can be induced to produce retrovirus-like particles. The induction procedures are the same as those previously shown to induce the synthesis of animal retroviruses. Electron microscopical evidence is presented that the human teratocarcinoma-derived (HTD) particles are most closely related to the type C retrovirus strains. HTD particles can be banded at 1.16 g/ml in linear sucrose gradients, the characteristic density for retroviruses, and subsequently be used for negative staining and fine structure analysis.