Expression of immunoglobulin heavy chain at a high level in the absence of a 
proposed immunoglobulin enhancer element in cis

Wabl, MR; Burrows, PD

doi:10.1073/pnas.81.8.2452

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Expression of immunoglobulin heavy chain at a high level in the absence of a proposed immunoglobulin enhancer element in cis

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Wabl, MR
Wabl Group, Friedrich Miescher Laboratory, Max Planck Society;

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Burrows, PD
Wabl Group, Friedrich Miescher Laboratory, Max Planck Society;

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Citation

Wabl, M., & Burrows, P. (1984). Expression of immunoglobulin heavy chain at a high level in the absence of a proposed immunoglobulin enhancer element in cis. Proceedings of the National Academy of Sciences of the United States of America, 81(8), 2452-2455. doi:10.1073/pnas.81.8.2452.

Cite as: https://hdl.handle.net/21.11116/0000-000C-A009-5

Abstract

The major intron between the J and C gene segments of the immunoglobulin heavy (H) chain locus contains an enhancer-like sequence, and it has been proposed that this enhancer is necessary to achieve high levels of H chain expression. We have isolated a subclone of the lymphoid pre-B cell line 18-81 that lacks this enhancer but nevertheless produces mu chain at the level characteristic of pre-B cells. Another subclone with a larger deletion does not produce mu chain, but upon fusion with a myeloma that does not produce any immunoglobulin chain, mu chain is expressed by the homolog from the pre-B subclone. The hybridoma lacks the proposed enhancer element in cis; nevertheless it produces as much mu chain as other plasma cell hybridomas. Therefore, this enhancer element is not obligatory for a high level of H chain production.