Photoactivatable Large Stokes Shift Fluorophores for Multicolor Nanoscopy

Likhotkin, Ilya; Lincoln, Richard; Bossi, Mariano Luis; Butkevich, Alexey; Hell, Stefan W.

doi:10.1021/jacs.2c12567

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学術論文

Photoactivatable Large Stokes Shift Fluorophores for Multicolor Nanoscopy

MPS-Authors

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Bossi, Mariano Luis
Department of NanoBiophotonics, Max Planck Institute for Multidisciplinary Sciences, Max Planck Society;

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Butkevich, Alexey
Department of NanoBiophotonics, Max Planck Institute for Multidisciplinary Sciences, Max Planck Society;

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Hell, Stefan W.
Department of NanoBiophotonics, Max Planck Institute for Multidisciplinary Sciences, Max Planck Society;

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引用

Likhotkin, I., Lincoln, R., Bossi, M. L., Butkevich, A., & Hell, S. W. (2023). Photoactivatable Large Stokes Shift Fluorophores for Multicolor Nanoscopy. Journal of the American Chemical Society, 145(3), 1530-1534. doi:10.1021/jacs.2c12567.

引用: https://hdl.handle.net/21.11116/0000-000C-AB01-2

要旨

We designed caging-group-free photoactivatable live-cell permeant dyes with red fluorescence emission and ∼100 nm Stokes shifts based on a 1-vinyl-10-silaxanthone imine core structure. The proposed fluorophores undergo byproduct-free one- and two-photon activation, are suitable for multicolor fluorescence microscopy in fixed and living cells, and are compatible with super-resolution techniques such as STED (stimulated emission depletion) and PALM (photoactivated localization microscopy). Use of photoactivatable labels for strain-promoted tetrazine ligation and self-labeling protein tags (HaloTag, SNAP-tag), and duplexing of an imaging channel with another large Stokes shift dye have been demonstrated.