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Shear rheology of methyl cellulose based solutions for cell mechanical measurements at high shear rates

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Büyükurganci,  Beyza
Max-Planck-Zentrum für Physik und Medizin, Max Planck Institute for the Science of Light, Max Planck Society;
Guck Division, Max Planck Institute for the Science of Light, Max Planck Society;

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Guck,  Jochen
Max-Planck-Zentrum für Physik und Medizin, Max Planck Institute for the Science of Light, Max Planck Society;
Guck Division, Max Planck Institute for the Science of Light, Max Planck Society;
Friedrich-Alexander-Universität Erlangen-Nürnberg;

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Reichel,  Felix
Guck Division, Max Planck Institute for the Science of Light, Max Planck Society;

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Soft Matter 2023 Bueyuekurganci.pdf
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Citation

Büyükurganci, B., Basu, S. K., Neuner, M., Guck, J., Wierschem, A., & Reichel, F. (2023). Shear rheology of methyl cellulose based solutions for cell mechanical measurements at high shear rates. Soft Matter, 19, 1739-1748. doi:10.1039/D2SM01515C.


Cite as: https://hdl.handle.net/21.11116/0000-000C-BBCE-A
Abstract
Methyl cellulose (MC) is a widely used material in various microfluidic applications in biology. Due to its biocompatibility, it has become a popular crowding agent for microfluidic cell deformability measurements, which usually operate at high shear rates (>10 000 s−1). However, a full rheological characterization of methyl cellulose solutions under these conditions has not yet been reported. With this study, we provide a full shear-rheological description for solutions of up to 1% MC dissolved in phosphate-buffered saline (PBS) that are commonly used in real-time deformability cytometry (RT-DC). We characterized three different MC-PBS solutions used for cell mechanical measurements in RT-DC with three different shear rheometer setups to cover a range of shear rates from 0.1–150 000 s−1. We report viscosities and normal stress differences in this regime. Viscosity functions can be well described using a Carreau–Yasuda model. Furthermore, we present the temperature dependency of shear viscosity and first normal stress difference of these solutions. Our results show that methyl cellulose solutions behave like power-law liquids in viscosity and exhibit first normal stress difference at shear rates between 5000–150 000 s−1. We construct a general viscosity equation for each MC solution at a certain shear rate and temperature. Furthermore, we investigated how MC concentration influences the rheology of the solutions and found the entanglement concentration at around 0.64 w/w%. Our results help to better understand the viscoelastic behavior of MC solutions, which can now be considered when modelling stresses in microfluidic channels.