Hello darkness, my old friend: 3-KETOACYL-COENZYME A SYNTHASE4 is a branch 
point in the regulation of triacylglycerol synthesis in Arabidopsis thaliana

Luzarowska, Urszula; Ruß, A.-K.; Joubès, Jérôme; Batsale, Marguerite; Szymański, Jędrzej; Thirumalaikumar, V. P.; Luzarowski, M.; Wu, Si; Zhu, F.; Endres, N.; Khedhayir, S.; Schumacher, Julia; Jasinska, Weronika; Xu, K.; Cordoba, Sandra Marcela Correa; Weil, Simy; Skirycz, A.; Fernie, A. R.; Li-Beisson, Yonghua; Fusari, Corina M; Brotman, Yariv

doi:10.1093/plcell/koad059

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Hello darkness, my old friend: 3-KETOACYL-COENZYME A SYNTHASE4 is a branch point in the regulation of triacylglycerol synthesis in Arabidopsis thaliana

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Ruß, A.-K.
Small-Molecule Signalling, Department Willmitzer, Max Planck Institute of Molecular Plant Physiology, Max Planck Society;

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Thirumalaikumar, V. P.
Stress Control Networks, Department Willmitzer, Max Planck Institute of Molecular Plant Physiology, Max Planck Society;

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Luzarowski, M.
Small Molecules, Department Willmitzer, Max Planck Institute of Molecular Plant Physiology, Max Planck Society;

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Zhu, F.
Central Metabolism, Department Gutjahr, Max Planck Institute of Molecular Plant Physiology, Max Planck Society;

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Endres, N.
Small Molecules, Department Willmitzer, Max Planck Institute of Molecular Plant Physiology, Max Planck Society;

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Khedhayir, S.
Genetics of Metabolic Traits, Cooperative Research Groups, Max Planck Institute of Molecular Plant Physiology, Max Planck Society;

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Xu, K.
Genetics of Metabolic Traits, Cooperative Research Groups, Max Planck Institute of Molecular Plant Physiology, Max Planck Society;

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Skirycz, A.
Small-Molecule Signalling, Department Willmitzer, Max Planck Institute of Molecular Plant Physiology, Max Planck Society;

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Fernie, A. R.
Central Metabolism, Department Gutjahr, Max Planck Institute of Molecular Plant Physiology, Max Planck Society;

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Citation

Luzarowska, U., Ruß, A.-K., Joubès, J., Batsale, M., Szymański, J., Thirumalaikumar, V. P., et al. (2023). Hello darkness, my old friend: 3-KETOACYL-COENZYME A SYNTHASE4 is a branch point in the regulation of triacylglycerol synthesis in Arabidopsis thaliana. The Plant Cell, 35(6), 1984-2005. doi:10.1093/plcell/koad059.

Cite as: https://hdl.handle.net/21.11116/0000-000C-B70B-A

Abstract

Plant lipids are important as alternative sources of carbon and energy when sugars or starch are limited. Here, we applied combined heat and darkness or extended darkness to a panel of ∼ 300 Arabidopsis (Arabidopsis thaliana) accessions to study lipid remodeling under carbon starvation. Natural allelic variation at 3-KETOACYL-COENZYME A SYNTHASE4 (KCS4), a gene encoding an enzyme involved in very long chain fatty-acid (VLCFA) synthesis, underlies the differential accumulation of polyunsaturated triacylglycerols (puTAGs) under stress. Ectopic expression of KCS4 in yeast and plants proved that KCS4 is a functional enzyme localized in the endoplasmic reticulum with specificity for C22 and C24 saturated acyl-CoA. Allelic mutants and transient overexpression in planta revealed the differential role of KCS4 alleles in VLCFA synthesis and leaf wax coverage, puTAG accumulation and biomass. Moreover, the region harboring KCS4 is under high selective pressure and allelic variation at KCS4 correlated with environmental parameters from the locales of Arabidopsis accessions. Our results provide evidence that KCS4 plays a decisive role in the subsequent fate of fatty acids released from chloroplast membrane lipids under carbon starvation. This work sheds light on both plant response mechanisms and the evolutionary events shaping the lipidome under carbon starvation.