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Transsynaptic labeling and transcriptional control of zebrafish neural circuits

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Roman,  I
Research Group Systems Neuroscience & Neuroengineering, Max Planck Institute for Biological Cybernetics, Max Planck Society;

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Li,  J       
Research Group Systems Neuroscience & Neuroengineering, Max Planck Institute for Biological Cybernetics, Max Planck Society;

/persons/resource/persons241750

Robson,  D       
Research Group Systems Neuroscience & Neuroengineering, Max Planck Institute for Biological Cybernetics, Max Planck Society;

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Citation

Coomer, C., Naumova, D., Talay, M., Zoliyom, M., Snell, N., Sorkaç, A., et al. (submitted). Transsynaptic labeling and transcriptional control of zebrafish neural circuits.


Cite as: https://hdl.handle.net/21.11116/0000-000C-E8AE-B
Abstract
Deciphering the connectome, the ensemble of synaptic connections that underlie brain function is a central goal of neuroscience research. The trans-Tango genetic approach, initially developed for anterograde transsynaptic tracing in Drosophila, can be used to map connections between presynaptic and postsynaptic partners and to drive gene expression in target neurons. Here, we describe the successful adaptation of trans-Tango to visualize neural connections in a living vertebrate nervous system, that of the zebrafish. Connections were validated between synaptic partners in the larval retina and brain. Results were corroborated by functional experiments in which optogenetic activation of retinal ganglion cells elicited responses in neurons of the optic tectum, as measured by trans-Tango-dependent expression of a genetically encoded calcium indicator. Transsynaptic signaling through trans-Tango reveals predicted as well as previously undescribed synaptic connections, providing a valuable in vivo tool to monitor and interrogate neural circuits over time.