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Journal Article

Sequence-specific DNA labelling for fluorescence microscopy

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Pradhan,  Shalini
Department of NanoBiophotonics, Max Planck Institute for Multidisciplinary Sciences, Max Planck Society;
Research Group of Chromatin Labeling and Imaging, Max Planck Institute for Multidisciplinary Sciences, Max Planck Society;

/persons/resource/persons288711

Apaydin,  Sinem
Department of NanoBiophotonics, Max Planck Institute for Multidisciplinary Sciences, Max Planck Society;
Research Group of Chromatin Labeling and Imaging, Max Planck Institute for Multidisciplinary Sciences, Max Planck Society;

/persons/resource/persons222860

Bucevičius,  Jonas
Department of NanoBiophotonics, Max Planck Institute for Multidisciplinary Sciences, Max Planck Society;
Research Group of Chromatin Labeling and Imaging, Max Planck Institute for Multidisciplinary Sciences, Max Planck Society;

/persons/resource/persons220611

Gerasimaitė,  Rūta
Department of NanoBiophotonics, Max Planck Institute for Multidisciplinary Sciences, Max Planck Society;
Research Group of Chromatin Labeling and Imaging, Max Planck Institute for Multidisciplinary Sciences, Max Planck Society;

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Kostiuk,  Georgij
Department of NanoBiophotonics, Max Planck Institute for Multidisciplinary Sciences, Max Planck Society;
Research Group of Chromatin Labeling and Imaging, Max Planck Institute for Multidisciplinary Sciences, Max Planck Society;

/persons/resource/persons203920

Lukinavičius,  Gražvydas
Department of NanoBiophotonics, Max Planck Institute for Multidisciplinary Sciences, Max Planck Society;
Research Group of Chromatin Labeling and Imaging, Max Planck Institute for Multidisciplinary Sciences, Max Planck Society;

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Citation

Pradhan, S., Apaydin, S., Bucevičius, J., Gerasimaitė, R., Kostiuk, G., & Lukinavičius, G. (2023). Sequence-specific DNA labelling for fluorescence microscopy. Biosensors and Bioelectronics, 230: 115256. doi:10.1016/j.bios.2023.115256.


Cite as: https://hdl.handle.net/21.11116/0000-000C-F984-6
Abstract
The preservation of nucleus structure during microscopy imaging is a top priority for understanding chromatin organization, genome dynamics, and gene expression regulation. In this review, we summarize the sequence-specific DNA labelling methods that can be used for imaging in fixed and/or living cells without harsh treatment and DNA denaturation: (i) hairpin polyamides, (ii) triplex-forming oligonucleotides, (iii) dCas9 proteins, (iv) transcription activator-like effectors (TALEs) and (v) DNA methyltransferases (MTases). All these techniques are capable of identifying repetitive DNA loci and robust probes are available for telomeres and centromeres, but visualizing single-copy sequences is still challenging. In our futuristic vision, we see gradual replacement of the historically important fluorescence in situ hybridization (FISH) by less invasive and non-destructive methods compatible with live cell imaging. Combined with super-resolution fluorescence microscopy, these methods will open the possibility to look into unperturbed structure and dynamics of chromatin in living cells, tissues and whole organisms.