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Journal Article

LIGHT-SABRE Hyperpolarizes 1-13C-Pyruvate Continuously without Magnetic Field Cycling


Stevanato,  Gabriele
Research Group of NMR Signal Enhancement, Max Planck Institute for Multidisciplinary Sciences, Max Planck Society;

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Pravdivtsev, A. N., Buckenmaier, K., Kempf, N., Stevanato, G., Scheffler, K., Engelmann, J., et al. (2023). LIGHT-SABRE Hyperpolarizes 1-13C-Pyruvate Continuously without Magnetic Field Cycling. The Journal of Physical Chemistry C, 127(14), 6744-6753. doi:10.1021/acs.jpcc.3c01128.

Cite as: https://hdl.handle.net/21.11116/0000-000D-0491-A
Nuclear spin hyperpolarization enables real-time observation of metabolism and intermolecular interactions in vivo. 1-13C-pyruvate is the leading hyperpolarized tracer currently under evaluation in several clinical trials as a promising molecular imaging agent. Still, the quest for a simple, fast, and efficient hyperpolarization technique is ongoing. Here, we describe that continuous, weak irradiation in the audio-frequency range of the 13C spin at the 121 μT magnetic field (approximately twice Earth’s field) enables spin order transfer from parahydrogen to 13C magnetization of 1-13C-pyruvate. These so-called LIGHT-SABRE pulses couple nuclear spin states of parahydrogen and pyruvate via the J-coupling network of reversibly exchanging Ir-complexes. Using ∼100% parahydrogen at ambient pressure, we polarized 51 mM 1-13C-pyruvate in the presence of 5.1 mM Ir-complex continuously and repeatedly to a polarization of 1.1% averaged over free and catalyst-bound pyruvate. The experiments were conducted at −8 °C, where almost exclusively bound pyruvate was observed, corresponding to an estimated 11% polarization on bound pyruvate. The obtained hyperpolarization levels closely match those obtained via SABRE-SHEATH under otherwise identical conditions. The creation of three different types of spin orders was observed: transverse 13C magnetization along the applied magnetic field, 13C z-magnetization along the main field B0, and 13C–1H zz-spin order. With a superconducting quantum interference device (SQUID) for detection, we found that the generated spin orders result from 1H–13C J-coupling interactions, which are not visible even with our narrow linewidth below 0.3 Hz and at −8 °C.