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Journal Article

Metal-Induced Energy Transfer (MIET) for Live-Cell Imaging with Fluorescent Proteins

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Guido,  Isabella
Laboratory for Fluid Physics, Pattern Formation and Biocomplexity, Max Planck Institute for Dynamics and Self-Organization, Max Planck Society;

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Turco,  Laura
Laboratory for Fluid Physics, Pattern Formation and Biocomplexity, Max Planck Institute for Dynamics and Self-Organization, Max Planck Society;

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acsnano.2c12372.pdf
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Citation

Hauke, L., Isbaner, S., Ghosh, A., Guido, I., Turco, L., Chizhik, A. I., et al. (2023). Metal-Induced Energy Transfer (MIET) for Live-Cell Imaging with Fluorescent Proteins. ACS Nano, 17(9), 8242-8251. doi:10.1021/acsnano.2c12372.


Cite as: https://hdl.handle.net/21.11116/0000-000D-06AA-D
Abstract
Metal-induced energy transfer (MIET) imaging is an easy-to-implement super-resolution modality that achieves nanometer resolution along the optical axis of a microscope. Although its capability in numerous biological and biophysical studies has been demonstrated, its implementation for live-cell imaging with fluorescent proteins is still lacking. Here, we present its applicability and capabilities for live-cell imaging with fluorescent proteins in diverse cell types (adult human stem cells, human osteo-sarcoma cells, and Dictyostelium discoideum cells), and with various fluorescent proteins (GFP, mScarlet, RFP, YPet). We show that MIET imaging achieves nanometer axial mapping of living cellular and subcellular components across multiple time scales, from a few milliseconds to hours, with negligible phototoxic effects.