Modular Assembly of Synthetic Secondary Chromosomes

Zumkeller, Celine; Schindler, Daniel; Waldminghaus, Torsten

doi:10.1007/978-1-4939-8675-0_5

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Modular Assembly of Synthetic Secondary Chromosomes

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引用

Zumkeller, C., Schindler, D., & Waldminghaus, T. (2018). Modular Assembly of Synthetic Secondary Chromosomes. In BACTERIAL CHROMATIN: Methods and Protocols (pp. 71-94).

引用: https://hdl.handle.net/21.11116/0000-000D-0781-9

要旨

The development of novel DNA assembly methods in recent years has paved
the way for the construction of synthetic replicons to be used for basic
research and biotechnological applications. Questions of how chromosomes
need to be constructed to maintain the genetic information can now be
answered by a learning-by-building approach. Here, we describe an
efficient pipeline for the design and assembly of synthetic, secondary
chromosomes in Escherichia coli based on the popular Modular Cloning
system (MoClo).