English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT

Released

Preprint

Enhanced assembly of bacteriophage T7 produced in cell-free reactions under simulated microgravity

MPS-Authors
/persons/resource/persons267088

Lehr,  Francois-Xavier
Emmy Noether research Group Cell-free Synthetic Biology, Max Planck Institute for Terrestrial Microbiology, Max Planck Society;

/persons/resource/persons261236

Glatter,  Timo       
Core Facility Mass Spectrometry and Proteomics, Max Planck Institute for Terrestrial Microbiology, Max Planck Society;

/persons/resource/persons261266

Niederholtmeyer,  Henrike       
Emmy Noether research Group Cell-free Synthetic Biology, Max Planck Institute for Terrestrial Microbiology, Max Planck Society;
external;

External Resource
Fulltext (restricted access)
There are currently no full texts shared for your IP range.
Fulltext (public)
There are no public fulltexts stored in PuRe
Supplementary Material (public)
There is no public supplementary material available
Citation

Lehr, F.-X., Pavletić, B., Glatter, T., Heimerl, T., Moeller, R., & Niederholtmeyer, H. (2022). Enhanced assembly of bacteriophage T7 produced in cell-free reactions under simulated microgravity. bioRxiv: the preprint server for biology, 2022.12.16.520761.


Cite as: https://hdl.handle.net/21.11116/0000-000D-0D56-5
Abstract
On-demand biomanufacturing has the potential to improve healthcare and self-sufficiency during space missions. Cell-free transcription and translation reactions combined with DNA blueprints can produce promising therapeutics like bacteriophages and virus-like particles. However, how space conditions affect the synthesis and self-assembly of such complex multi-protein structures is unknown. Here, we characterize the cell-free production of infectious bacteriophage T7 virions under simulated microgravity. Rotation in a 2D-clinostat increased the number of infectious particles compared to static controls. Quantitative analyses by mass spectrometry, immuno-dot-blot and real-time PCR showed no significant differences in protein and DNA contents, suggesting enhanced self-assembly of T7 phages in simulated microgravity. While the effects of genuine space conditions on the cell-free synthesis and assembly of bacteriophages remain to be investigated, our findings support the vision of a cell-free synthesis-enabled “astropharmacy”.Competing Interest StatementThe authors have declared no competing interest.