Specific, sensitive and quantitative protein detection by in-gel fluorescence

Fuchs, ACD

doi:10.1038/s41467-023-38147-8

Item

ITEM ACTIONSEXPORT

Add to Basket

Local TagsRelease HistoryDetailsSummary

Released

Journal Article

Specific, sensitive and quantitative protein detection by in-gel fluorescence

MPS-Authors

/persons/resource/persons272125

Fuchs, ACD
Department Protein Evolution, Max Planck Institute for Biology Tübingen, Max Planck Society;

External Resource

No external resources are shared

Fulltext (restricted access)

There are currently no full texts shared for your IP range.

Fulltext (public)

There are no public fulltexts stored in PuRe

Supplementary Material (public)

There is no public supplementary material available

Citation

Fuchs, A. (2023). Specific, sensitive and quantitative protein detection by in-gel fluorescence. Nature Communications, 14(1): 2505. doi:10.1038/s41467-023-38147-8.

Cite as: https://hdl.handle.net/21.11116/0000-000D-0F93-D

Abstract

Recombinant proteins in complex solutions are typically detected with tag-specific antibodies in Western blots. Here we describe an antibody-free alternative in which tagged proteins are detected directly in polyacrylamide gels. For this, the highly specific protein ligase Connectase is used to selectively fuse fluorophores to target proteins carrying a recognition sequence, the CnTag. Compared to Western blots, this procedure is faster, more sensitive, offers a better signal-to-noise ratio, requires no optimization for different samples, allows more reproducible and accurate quantifications, and uses freely available reagents. With these advantages, this method represents a promising alternative to the state of the art and may facilitate studies on recombinant proteins.