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Sample Preparation for Time-Resolved Serial Crystallography: Practical Considerations

MPS-Authors
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Mehrabi,  P.
Institute for Nanostructure and Solid State Physics, Universität Hamburg;
Miller Group, Atomically Resolved Dynamics Department, Max Planck Institute for the Structure and Dynamics of Matter, Max Planck Society;

/persons/resource/persons202768

Schulz,  E.-C.
Miller Group, Atomically Resolved Dynamics Department, Max Planck Institute for the Structure and Dynamics of Matter, Max Planck Society;
University Medical Center Hamburg-Eppendorf (UKE);

External Resource

https://doi.org/10.1007/978-1-0716-3147-8_21
(Publisher version)

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Citation

Mehrabi, P., & Schulz, E.-C. (2023). Sample Preparation for Time-Resolved Serial Crystallography: Practical Considerations. In A. Sousa, & L. Passarinha (Eds.), Advanced Methods in Structural Biology (pp. 361-379). Berlin/Heidelberg: Springer Science+Business Media. doi:10.1007/978-1-0716-3147-8_21.


Cite as: https://hdl.handle.net/21.11116/0000-000D-10F5-C
Abstract
Time-resolved serial crystallography is an emerging method to elucidate the structure-function relationship of biomolecular systems at up to atomic resolution. However, to make this demanding method a success, a number of experimental requirements have to be met. In this chapter, we summarize general guidelines and protocols towards performing time-resolved crystallography experiments, with a particular emphasis on sample requirements and preparation but also a brief excursion into reaction initiation.