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The wet plate protocol: an efficient way to obtain dauer larvae

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Weller,  A
Department Integrative Evolutionary Biology, Max Planck Institute for Developmental Biology, Max Planck Society;

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Citation

Weller, A. (2010). The wet plate protocol: an efficient way to obtain dauer larvae. The Worm Breeder’s Gazette, 18(3).


Cite as: https://hdl.handle.net/21.11116/0000-000D-1263-F
Abstract
Developmentally arrested dauer stages in nematodes for laboratory assays have so far been obtained either by simply starving culture plates or by liquid culture in large Erlenmeyer flasks. Harvesting dauers from starved agar plates is straightforward but the yield is usually very low. Liquid cultures on the other hand produce high yields, but their maintenance is labor-intensive. We have combined the simple setup of starved plates with the increased yield of liquid cultures in the “wet plate protocol”.