Help Privacy Policy Disclaimer
  Advanced SearchBrowse




Journal Article

The wet plate protocol: an efficient way to obtain dauer larvae


Weller,  A
Department Integrative Evolutionary Biology, Max Planck Institute for Developmental Biology, Max Planck Society;

Fulltext (restricted access)
There are currently no full texts shared for your IP range.
Fulltext (public)
There are no public fulltexts stored in PuRe
Supplementary Material (public)
There is no public supplementary material available

Weller, A. (2010). The wet plate protocol: an efficient way to obtain dauer larvae. The Worm Breeder’s Gazette, 18(3).

Cite as: https://hdl.handle.net/21.11116/0000-000D-1263-F
Developmentally arrested dauer stages in nematodes for laboratory assays have so far been obtained either by simply starving culture plates or by liquid culture in large Erlenmeyer flasks. Harvesting dauers from starved agar plates is straightforward but the yield is usually very low. Liquid cultures on the other hand produce high yields, but their maintenance is labor-intensive. We have combined the simple setup of starved plates with the increased yield of liquid cultures in the “wet plate protocol”.