Effects of detergents, proteins, and lipids on 2':3'-cyclic-nucleotide 
3'-phosphodiesterase activity

Clapshaw, PA; Seifert, W

doi:10.1111/j.1471-4159.1980.tb12502.x

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Effects of detergents, proteins, and lipids on 2':3'-cyclic-nucleotide 3'-phosphodiesterase activity

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Clapshaw, PA
Seifert Group, Friedrich Miescher Laboratory, Max Planck Society;

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Seifert, W
Seifert Group, Friedrich Miescher Laboratory, Max Planck Society;

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Citation

Clapshaw, P., & Seifert, W. (1980). Effects of detergents, proteins, and lipids on 2':3'-cyclic-nucleotide 3'-phosphodiesterase activity. Journal of Neurochemistry, 35(1), 164-169. doi:10.1111/j.1471-4159.1980.tb12502.x.

Cite as: https://hdl.handle.net/21.11116/0000-000D-2E63-1

Abstract

The myelin marker 2':3'-cyclic-nucleotide 34'-phosphodiesterase (CNPase) was isolated to a lipid- and phosphate-free stage. The effects of exogenously added lipids were tested on this preparation and compared to the known stimulation of the enzyme by detergents and proteins. CNPase could be stimulated 2-3 fold by these various agents which appeared to be additive in their effect. Enzyme-protein and enzyme-lipid interactions and possible medical use of the improved assay conditions for CNPase employed in the study are discussed.