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Journal Article

Intracellular localization of 2',3'-cyclic nucleotide 3'-phosphodiesterase in a neuronal cell line as examined by immunofluorescence and cell fractionation

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Müller,  HW
Seifert Group, Friedrich Miescher Laboratory, Max Planck Society;

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Clapshaw,  PA
Seifert Group, Friedrich Miescher Laboratory, Max Planck Society;

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Seifert,  W
Seifert Group, Friedrich Miescher Laboratory, Max Planck Society;

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Citation

Müller, H., Clapshaw, P., & Seifert, W. (1981). Intracellular localization of 2',3'-cyclic nucleotide 3'-phosphodiesterase in a neuronal cell line as examined by immunofluorescence and cell fractionation. Journal of Neurochemistry, 37(4), 947-955. doi:10.1111/j.1471-4159.1981.tb04482.x.


Cite as: https://hdl.handle.net/21.11116/0000-000D-2E6B-9
Abstract
The enzyme 2',3'-cyclic nucleotide 3'-phosphodiesterase (CNPase, EC 3.1.4.37) occurs not only in myelin fractions and glial cells, but can also be shown to be present in a CNS cell line of neuronal origin (B104). Direct immunofluorescence microscopy of B104 cells with fluorescein isothiocyanate-conjugated rabbit anti-CNPase antibodies shows a discrete and specific intracytoplasmic location of CNPase. Fractionation of the cells was performed by differential centrifugation of a cell homogenate and continuous sucrose density-gradient centrifugation. As monitored by marker enzyme activities, CNPase seems to be associated with endoplasmic reticulum membranes.