English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT

Released

Journal Article

Involvement of chitin in exoskeleton morphogenesis in Drosophila melanogaster

MPS-Authors
/persons/resource/persons214650

Moussian,  B       
Department Genetics, Max Planck Institute for Developmental Biology, Max Planck Society;

/persons/resource/persons272672

Schwarz,  H
Electron Microscopy, Max Planck Institute for Developmental Biology, Max Planck Society;

/persons/resource/persons285843

Bartoszewski,  S       
Department Genetics, Max Planck Institute for Developmental Biology, Max Planck Society;

/persons/resource/persons271460

Nüsslein-Volhard,  C       
Department Genetics, Max Planck Institute for Developmental Biology, Max Planck Society;

External Resource
No external resources are shared
Fulltext (restricted access)
There are currently no full texts shared for your IP range.
Fulltext (public)
There are no public fulltexts stored in PuRe
Supplementary Material (public)
There is no public supplementary material available
Citation

Moussian, B., Schwarz, H., Bartoszewski, S., & Nüsslein-Volhard, C. (2005). Involvement of chitin in exoskeleton morphogenesis in Drosophila melanogaster. Journal of Morphology, 264(1), 117-130. doi:10.1002/jmor.10324.


Cite as: https://hdl.handle.net/21.11116/0000-000D-2FB6-2
Abstract
Exoskeletons stabilize cell, tissue, and body morphology in many living organisms including fungi, plants, and arthropods. In insects, the exoskeleton, the cuticle, is produced by epidermal cells as a protein extracellular matrix containing lipids and the polysaccharide chitin, and its formation requires coordinated synthesis, distribution, and modification of these components. Eventually, the stepwise secretion and sorting of the cuticle material results in a layered structure comprising the envelope, the proteinaceous epicuticle, and the chitinous procuticle. To study the role of chitin during cuticle development, we analyzed the consequences of chitin absence in the embryo of Drosophila melanogaster caused by mutations in the Chitin Synthase-1 (CS-1) gene, called krotzkopf verkehrt (kkv). Our histological data confirm that chitin is essential for procuticle integrity and further demonstrate that an intact procuticle is important to assemble and to stabilize the chitin-less epicuticle. Moreover, the phenotype of CS-1/kkv mutant embryos indicates that chitin is required to attach the cuticle to the epidermal cells, thereby maintaining epidermal morphology. Finally, sclerotization and pigmentation, which are the last steps in cuticle differentiation, are impaired in tissues lacking CS-1/kkv function, suggesting that proper cuticle structure is crucial for the activity of the underlying enzymes.