A simple flow-through micro-chamber for handling fragile, small tissue explants 
and single non-adherent cells

Hausen, P; Riebesell, M

doi:10.1023/a:1024445010789

Item

ITEM ACTIONSEXPORT

Add to Basket

Local TagsRelease HistoryDetailsSummary

Released

Journal Article

A simple flow-through micro-chamber for handling fragile, small tissue explants and single non-adherent cells

MPS-Authors

/persons/resource/persons274234

Hausen, P
Department Cell Biology, Max Planck Institute for Developmental Biology, Max Planck Society;

/persons/resource/persons272119

Riebesell, M
Department Cell Biology, Max Planck Institute for Developmental Biology, Max Planck Society;

External Resource

No external resources are shared

Fulltext (restricted access)

There are currently no full texts shared for your IP range.

Fulltext (public)

There are no public fulltexts stored in PuRe

Supplementary Material (public)

There is no public supplementary material available

Citation

Hausen, P., & Riebesell, M. (2002). A simple flow-through micro-chamber for handling fragile, small tissue explants and single non-adherent cells. Methods in Cell Science, 24(4), 165-168. doi:10.1023/a:1024445010789.

Cite as: https://hdl.handle.net/21.11116/0000-000D-484E-C

Abstract

The flow-through chambers described are easily assembled in the laboratory with no need for sophisticated or expensive equipment. They allow for treating micro-explants or single non-adhesive cells with different solutes for defined times by easy and quick changes of the medium in the chambers. The specimen can be fixed, immunostained and observed microscopically within the chamber. During these procedures the cells are not exposed to undue mechanical stress and fragile intercellular structures are well preserved.