Deutsch
 
Hilfe Datenschutzhinweis Impressum
  DetailsucheBrowse
  1. Datensatz ansehen / 
  2. Datensatz Übersicht

Datensatz

DATENSATZ AKTIONENEXPORT
Zur Ablage hinzufügen
  Lokale TagsFreigabegeschichteDetailsÜbersicht

Freigegeben
Zeitschriftenartikel

Translation dynamics in human cells visualized at high resolution reveal cancer drug action

MPG-Autoren
/persons/resource/persons290857

Xing,  Huaipeng       
Department of Molecular Sociology, Max Planck Institute of Biophysics, Max Planck Society;
Faculty of Biochemistry, Chemistry and Pharmacy, Goethe University Frankfurt am Main, Frankfurt am Main, Germany;

/persons/resource/persons275016

Taniguchi,  Reiya       
Department of Molecular Sociology, Max Planck Institute of Biophysics, Max Planck Society;

/persons/resource/persons248009

Khusainov,  Iskander       
Department of Molecular Sociology, Max Planck Institute of Biophysics, Max Planck Society;

/persons/resource/persons284138

Kreysing,  Jan Philipp       
Department of Molecular Sociology, Max Planck Institute of Biophysics, Max Planck Society;
IMPRS-CBP, Max Planck Institute of Biophysics, Max Planck Society;

/persons/resource/persons250130

Welsch,  Sonja       
Central Electron Microscopy Facility, Max Planck Institute of Biophysics, Max Planck Society;

/persons/resource/persons188929

Turoňová,  Beata       
Department of Molecular Sociology, Max Planck Institute of Biophysics, Max Planck Society;

/persons/resource/persons232071

Beck,  Martin       
Department of Molecular Sociology, Max Planck Institute of Biophysics, Max Planck Society;
Institute of Biochemistry, Goethe University Frankfurt, Frankfurt am Main, Germany;

Externe Ressourcen
Es sind keine externen Ressourcen hinterlegt
Volltexte (beschränkter Zugriff)
Für Ihren IP-Bereich sind aktuell keine Volltexte freigegeben.
Volltexte (frei zugänglich)
Ergänzendes Material (frei zugänglich)
Es sind keine frei zugänglichen Ergänzenden Materialien verfügbar
Zitation

Xing, H., Taniguchi, R., Khusainov, I., Kreysing, J. P., Welsch, S., Turoňová, B., et al. (2023). Translation dynamics in human cells visualized at high resolution reveal cancer drug action. Science, 381(6653), 70-75. doi:10.1126/science.adh1411.


Zitierlink: https://hdl.handle.net/21.11116/0000-000D-6B03-8
Zusammenfassung
Ribosomes catalyze protein synthesis by cycling through various functional states. These states have been extensively characterized in vitro, but their distribution in actively translating human cells remains elusive. We used a cryo-electron tomography-based approach and resolved ribosome structures inside human cells with high resolution. These structures revealed the distribution of functional states of the elongation cycle, a Z transfer RNA binding site, and the dynamics of ribosome expansion segments. Ribosome structures from cells treated with Homoharringtonine, a drug used against chronic myeloid leukemia, revealed how translation dynamics were altered in situ and resolve the small molecules within the active site of the ribosome. Thus, structural dynamics and drug effects can be assessed at high resolution within human cells.