Molecular basis and design principles of switchable front-rear polarity and 
directional migration in Myxococcus xanthus

Carreira, Luis Antonio Menezes; Szadkowski, Dobromir; Lometto, Stefano; Hochberg, Georg K. A.; Søgaard-Andersen, Lotte

doi:10.1038/s41467-023-39773-y

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Molecular basis and design principles of switchable front-rear polarity and directional migration in Myxococcus xanthus

MPS-Authors

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Carreira, Luis Antonio Menezes
Bacterial Adaption and Differentiation, Department of Ecophysiology, Max Planck Institute for Terrestrial Microbiology, Max Planck Society;

/persons/resource/persons254751

Szadkowski, Dobromir
Bacterial Adaption and Differentiation, Department of Ecophysiology, Max Planck Institute for Terrestrial Microbiology, Max Planck Society;

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Lometto, Stefano
Max Planck Research Group Evolutionary Biochemistry, Max Planck Institute for Terrestrial Microbiology, Max Planck Society;

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Hochberg, Georg K. A.
Max Planck Research Group Evolutionary Biochemistry, Max Planck Institute for Terrestrial Microbiology, Max Planck Society;

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Søgaard-Andersen, Lotte
Bacterial Adaption and Differentiation, Department of Ecophysiology, Max Planck Institute for Terrestrial Microbiology, Max Planck Society;

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https://doi.org/10.1038/s41467-023-39773-y
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引用

Carreira, L. A. M., Szadkowski, D., Lometto, S., Hochberg, G. K. A., & Søgaard-Andersen, L. (2023). Molecular basis and design principles of switchable front-rear polarity and directional migration in Myxococcus xanthus. Nature Communications, 14(1):. doi:10.1038/s41467-023-39773-y.

引用: https://hdl.handle.net/21.11116/0000-000D-6B26-1

要旨

During cell migration, front-rear polarity is spatiotemporally regulated; however, the underlying design of regulatory interactions varies. In rod-shaped Myxococcus xanthus cells, a spatial toggle switch dynamically regulates front-rear polarity. The polarity module establishes front-rear polarity by guaranteeing front pole-localization of the small GTPase MglA. Conversely, the Frz chemosensory system, by acting on the polarity module, causes polarity inversions. MglA localization depends on the RomR/RomX GEF and MglB/RomY GAP complexes that localize asymmetrically to the poles by unknown mechanisms. Here, we show that RomR and the MglB and MglC roadblock domain proteins generate a positive feedback by forming a RomR/MglC/MglB complex, thereby establishing the rear pole with high GAP activity that is non-permissive to MglA. MglA at the front engages in negative feedback that breaks the RomR/MglC/MglB positive feedback allosterically, thus ensuring low GAP activity at this pole. These findings unravel the design principles of a system for switchable front-rear polarity.