RIBOSOMAL-RNA AND RDNA SEQUENCE ANALYSES

Stackebrandt, E; Liesack, W; Witt, D

doi:10.1016/0378-1119(92)90567-9

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RIBOSOMAL-RNA AND RDNA SEQUENCE ANALYSES

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Stackebrandt, E., Liesack, W., & Witt, D. (1992). RIBOSOMAL-RNA AND RDNA SEQUENCE ANALYSES. GENE, 115(1-2), 255-260. doi:10.1016/0378-1119(92)90567-9.

Cite as: https://hdl.handle.net/21.11116/0000-000D-6BB7-D

Abstract

The potential of ribosomal (r) RNA and the encoding genes (rDNA) to
elucidate natural relationships has been dramatically extended by
improved sequencing approaches and the application of polymerase chain
reaction. Sequence information on 16S and 23S rRNA/DNA from 69 strains
of 53 Streptomyces species allows determination of regions that can be
used as target sites for diagnostic probes, and for amplification and
sequencing primers. To generate phylogenetic trees, sequence
similarities are converted into distance values. The topologies of the
trees based on different parts of the molecule are compared among each
other and to the numerical phenotypic clustering of the strains.