The identification of genes with unique and essential functions in the 
development of the zebrafish, Danio rerio

Haffter, P; Granato, M; Brand, M; Mullins, MC; Hammerschmidt, M; Kane, DA; Odenthal, J; van Eeden, FJM; Jiang, Y-J; Heisenberg, C-P; Kelsh, RN; Furutani-Seiki, M; Vogelsang, E; Beuchle, D; Schach, U; Fabian, C; Nüsslein-Volhard, C

doi:10.1242/dev.123.1.1

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The identification of genes with unique and essential functions in the development of the zebrafish, Danio rerio

MPG-Autoren

/persons/resource/persons274411

Haffter, P
Department Genetics, Max Planck Institute for Developmental Biology, Max Planck Society;

/persons/resource/persons274425

Granato, M
Department Genetics, Max Planck Institute for Developmental Biology, Max Planck Society;

/persons/resource/persons219033

Brand, M
Department Genetics, Max Planck Institute for Developmental Biology, Max Planck Society;

/persons/resource/persons274439

Mullins, MC
Department Genetics, Max Planck Institute for Developmental Biology, Max Planck Society;

/persons/resource/persons191086

Hammerschmidt, M
Department Genetics, Max Planck Institute for Developmental Biology, Max Planck Society;

/persons/resource/persons274435

Kane, DA
Department Genetics, Max Planck Institute for Developmental Biology, Max Planck Society;

/persons/resource/persons274416

Odenthal, J
Department Genetics, Max Planck Institute for Developmental Biology, Max Planck Society;

/persons/resource/persons274409

van Eeden, FJM
Department Genetics, Max Planck Institute for Developmental Biology, Max Planck Society;

/persons/resource/persons274433

Jiang, Y-J
Department Genetics, Max Planck Institute for Developmental Biology, Max Planck Society;

/persons/resource/persons219231

Heisenberg, C-P
Department Genetics, Max Planck Institute for Developmental Biology, Max Planck Society;

/persons/resource/persons274437

Kelsh, RN
Department Genetics, Max Planck Institute for Developmental Biology, Max Planck Society;

/persons/resource/persons274429

Furutani-Seiki, M
Department Genetics, Max Planck Institute for Developmental Biology, Max Planck Society;

/persons/resource/persons274482

Vogelsang, E
Department Genetics, Max Planck Institute for Developmental Biology, Max Planck Society;

/persons/resource/persons277769

Beuchle, D
Department Genetics, Max Planck Institute for Developmental Biology, Max Planck Society;

/persons/resource/persons274034

Schach, U
Department Genetics, Max Planck Institute for Developmental Biology, Max Planck Society;

/persons/resource/persons290892

Fabian, C
Department Genetics, Max Planck Institute for Developmental Biology, Max Planck Society;

/persons/resource/persons271460

Nüsslein-Volhard, C
Department Genetics, Max Planck Institute for Developmental Biology, Max Planck Society;

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Zitation

Haffter, P., Granato, M., Brand, M., Mullins, M., Hammerschmidt, M., Kane, D., et al. (1996). The identification of genes with unique and essential functions in the development of the zebrafish, Danio rerio. Development, 123(1), 1-36. doi:10.1242/dev.123.1.1.

Zitierlink: https://hdl.handle.net/21.11116/0000-000D-6D54-B

Zusammenfassung

In a large-scale screen, we isolated mutants displaying a specific visible phenotype in embryos or early larvae of the zebrafish, Danio rerio. Males were mutagenized with ethylnitrosourea (ENU) and F2 families of single pair matings between sibling F1 fish, heterozygous for a mutagenized genome, were raised. Egg lays were obtained from several crosses between F2 siblings, resulting in scoring of 3857 mutagenized genomes. F3 progeny were scored at the second, third and sixth day of development, using a stereomicroscope. In a subsequent screen, fixed embryos were analyzed for correct retinotectal projection. A total of 4264 mutants were identified. Two thirds of the mutants displaying rather general abnormalities were eventually discarded. We kept and characterized 1163 mutants. In complementation crosses performed between mutants with similar phenotypes, 894 mutants have been assigned to 372 genes. The average allele frequency is 2.4. We identified genes involved in early development, notochord, brain, spinal cord, somites, muscles, heart, circulation, blood, skin, fin, eye, otic vesicle, jaw and branchial arches, pigment pattern, pigment formation, gut, liver, motility and touch response. Our collection contains alleles of almost all previously described zebrafish mutants. From the allele frequencies and other considerations we estimate that the 372 genes defined by the mutants probably represent more than half of all genes that could have been discovered using the criteria of our screen. Here we give an overview of the spectrum of mutant phenotypes obtained, and discuss the limits and the potentials of a genetic saturation screen in the zebrafish.