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Journal Article

Mutations in zebrafish genes affecting the formation of the boundary between midbrain and hindbrain

MPS-Authors
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Brand,  M       
Department Genetics, Max Planck Institute for Developmental Biology, Max Planck Society;

/persons/resource/persons219231

Heisenberg,  C-P
Department Genetics, Max Planck Institute for Developmental Biology, Max Planck Society;

/persons/resource/persons274433

Jiang,  Y-J
Department Genetics, Max Planck Institute for Developmental Biology, Max Planck Society;

/persons/resource/persons277769

Beuchle,  D
Department Genetics, Max Planck Institute for Developmental Biology, Max Planck Society;

/persons/resource/persons290961

Lun,  K
Department Genetics, Max Planck Institute for Developmental Biology, Max Planck Society;

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Furutani-Seiki,  M
Department Genetics, Max Planck Institute for Developmental Biology, Max Planck Society;

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Granato,  M
Department Genetics, Max Planck Institute for Developmental Biology, Max Planck Society;

/persons/resource/persons274411

Haffter,  P
Department Genetics, Max Planck Institute for Developmental Biology, Max Planck Society;

/persons/resource/persons191086

Hammerschmidt,  M
Department Genetics, Max Planck Institute for Developmental Biology, Max Planck Society;

/persons/resource/persons274435

Kane,  DA
Department Genetics, Max Planck Institute for Developmental Biology, Max Planck Society;

/persons/resource/persons274437

Kelsh,  RN       
Department Genetics, Max Planck Institute for Developmental Biology, Max Planck Society;

/persons/resource/persons274439

Mullins,  MC
Department Genetics, Max Planck Institute for Developmental Biology, Max Planck Society;

/persons/resource/persons274416

Odenthal,  J
Department Genetics, Max Planck Institute for Developmental Biology, Max Planck Society;

/persons/resource/persons274409

van Eeden,  FJ
Department Genetics, Max Planck Institute for Developmental Biology, Max Planck Society;

/persons/resource/persons271460

Nüsslein-Volhard,  C       
Department Genetics, Max Planck Institute for Developmental Biology, Max Planck Society;

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Citation

Brand, M., Heisenberg, C.-P., Jiang, Y.-J., Beuchle, D., Lun, K., Furutani-Seiki, M., et al. (1996). Mutations in zebrafish genes affecting the formation of the boundary between midbrain and hindbrain. Development, 123(1), 179-190. doi:10.1242/dev.123.1.179.


Cite as: https://hdl.handle.net/21.11116/0000-000D-6F79-0
Abstract
Mutations in two genes affect the formation of the boundary between midbrain and hindbrain (MHB): no isthmus (noi) and acerebellar (ace). noi mutant embryos lack the MHB constriction, the cerebellum and optic tectum, as well as the pronephric duct. Analysis of noi mutant embryos with neuron-specific antibodies shows that the MHB region and the dorsal and ventral midbrain are absent or abnormal, but that the rostral hindbrain is unaffected with the exception of the cerebellum. Using markers that are expressed during its formation (eng, wnt1 and pax-b), we find that the MHB region is already misspecified in noi mutant embryos during late gastrulation. The tectum is initially present and later degenerates. The defect in ace mutant embryos is more restricted: MHB and cerebellum are absent, but a tectum is formed. Molecular organisation of the tectum and tegmentum is disturbed, however, since eng, wnt1 and pax-b marker gene expression is not maintained. We propose that noi and ace are required for development of the MHB region and of the adjacent mid- and hindbrain, which are thought to be patterned by the MHB region. Presence of pax-b RNA, and absence of pax-b protein, together with the observation of genetic linkage and the occurrence of a point mutation, show that noi mutations are located in the pax-b gene. pax-b is a vertebrate orthologue of the Drosophila gene paired, which is involved in a pathway of cellular interactions at the posterior compartment boundary in Drosophila. Our results confirm and extend a previous report, and show that at least one member of this conserved signalling pathway is required for formation of the boundary between midbrain and hindbrain in the zebrafish.