English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
Add to Basket
  Local TagsRelease HistoryDetailsSummary

Released
Journal Article

The Mediator complex regulates enhancer-promoter interactions

MPS-Authors
/persons/resource/persons291663

Ramasamy,  S.
Lise Meitner Group Genome Organization and Regulation, Max Planck Institute for Multidisciplinary Sciences, Max Planck Society;

/persons/resource/persons252095

Aljahani,  Abrar
Lise Meitner Group Genome Organization and Regulation, Max Planck Institute for Multidisciplinary Sciences, Max Planck Society;

/persons/resource/persons252097

Karpinska,  Magdalena A.       
Lise Meitner Group Genome Organization and Regulation, Max Planck Institute for Multidisciplinary Sciences, Max Planck Society;

/persons/resource/persons291665

Cao,  Ngoc Tran Bich
Lise Meitner Group Genome Organization and Regulation, Max Planck Institute for Multidisciplinary Sciences, Max Planck Society;

/persons/resource/persons248339

Velychko,  Taras       
Department of Molecular Biology, Max Planck Institute for Multidisciplinary Sciences, Max Planck Society;

/persons/resource/persons291667

Cruz,  J. Neos
Lise Meitner Group Genome Organization and Regulation, Max Planck Institute for Multidisciplinary Sciences, Max Planck Society;

/persons/resource/persons146864

Lidschreiber,  Michael       
Department of Molecular Biology, Max Planck Institute for Multidisciplinary Sciences, Max Planck Society;

/persons/resource/persons252091

Oudelaar,  Aukje Marieke       
Lise Meitner Group Genome Organization and Regulation, Max Planck Institute for Multidisciplinary Sciences, Max Planck Society;

External Resource
No external resources are shared
Fulltext (restricted access)
There are currently no full texts shared for your IP range.
Fulltext (public)

s41594-023-01027-2.pdf
(Publisher version), 12MB

Supplementary Material (public)
There is no public supplementary material available
Citation

Ramasamy, S., Aljahani, A., Karpinska, M. A., Cao, N. T. B., Velychko, T., Cruz, J. N., et al. (2023). The Mediator complex regulates enhancer-promoter interactions. Nature Structural and Molecular Biology, 30, 991-1000. doi:10.1038/s41594-023-01027-2.


Cite as: https://hdl.handle.net/21.11116/0000-000D-955C-4
Abstract
Enhancer-mediated gene activation generally requires physical proximity between enhancers and their target gene promoters. However, the molecular mechanisms by which interactions between enhancers and promoters are formed are not well understood. Here, we investigate the function of the Mediator complex in the regulation of enhancer-promoter interactions, by combining rapid protein depletion and high-resolution MNase-based chromosome conformation capture approaches. We show that depletion of Mediator leads to reduced enhancer-promoter interaction frequencies, which are associated with a strong decrease in gene expression. In addition, we find increased interactions between CTCF-binding sites upon Mediator depletion. These changes in chromatin architecture are associated with a redistribution of the Cohesin complex on chromatin and a reduction in Cohesin occupancy at enhancers. Together, our results indicate that the Mediator and Cohesin complexes contribute to enhancer-promoter interactions and provide insights into the molecular mechanisms by which communication between enhancers and promoters is regulated.