Time-resolved Small-RNA Sequencing Identifies MicroRNAs Critical for Formation 
of Embryonic Stem Cells from the Inner Cell Mass of Mouse Embryos

Moradi, Sharif; Guenther, Stefan; Soori, Samira; Sharifi-Zarchi , Ali; Kuenne, Carsten; Khoddami , Vahid; Tavakol , Pouya; Kreutzer, Susanne; Braun, Thomas; Baharvand, Hossein

doi:10.1007/s12015-023-10582-6

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Time-resolved Small-RNA Sequencing Identifies MicroRNAs Critical for Formation of Embryonic Stem Cells from the Inner Cell Mass of Mouse Embryos

MPS-Authors

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Guenther, Stefan
Cardiac Development and Remodeling, Max Planck Institute for Heart and Lung Research, Max Planck Society;

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Kuenne, Carsten
Bioinformatics, Max Planck Institute for Heart and Lung Research, Max Planck Society;

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Kreutzer, Susanne
Max Planck Institute for Heart and Lung Research, Max Planck Society;

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Braun, Thomas
Cardiac Development and Remodeling, Max Planck Institute for Heart and Lung Research, Max Planck Society;

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引用

Moradi, S., Guenther, S., Soori, S., Sharifi-Zarchi, A., Kuenne, C., Khoddami, V., Tavakol, P., Kreutzer, S., Braun, T., & Baharvand, H. (2023). Time-resolved Small-RNA Sequencing Identifies MicroRNAs Critical for Formation of Embryonic Stem Cells from the Inner Cell Mass of Mouse Embryos. STEM CELL REVIEWS AND REPORTS. doi:10.1007/s12015-023-10582-6.

引用: https://hdl.handle.net/21.11116/0000-000D-7EB5-A

要旨

Cells of the inner cell mass (ICM) acquire a unique ability for unlimited self-renewal during transition into embryonic stem cells (ESCs) in vitro, while preserving their natural multi-lineage differentiation potential. Several different pathways have been identified to play roles in ESC formation but the function of non-coding RNAs in this process is poorly understood. Here, we describe several microRNAs (miRNAs) that are crucial for efficient generation of mouse ESCs from ICMs. Using small-RNA sequencing, we characterize dynamic changes in miRNA expression profiles during outgrowth of ICMs in a high-resolution, time-course dependent manner. We report several waves of miRNA transcription during ESC formation, to which miRNAs from the imprinted Dlk1-Dio3 locus contribute extensively. In silico analyses followed by functional investigations reveal that Dlk1-Dio3 locus-embedded miRNAs (miR-541-5p, miR-410-3p, and miR-381-3p), miR-183-5p, and miR-302b-3p promote, while miR-212-5p and let-7d-3p inhibit ESC formation. Collectively, these findings offer new mechanistic insights into the role of miRNAs during ESC derivation.