Two NLR immune receptors acquired high-affinity binding to a fungal effector 
through convergent evolution of their integrated domain

Białas, A; Langner, T; Harant, A; Contreras, MP; Stevenson, CE; Lawson, DM; Sklenar, J; Kellner, R; Moscou, MJ; Terauchi, R; Banfield, MJ; Kamoun, S

doi:10.7554/eLife.66961

Item

ITEM ACTIONSEXPORT

Add to Basket

Local TagsRelease HistoryDetailsSummary

Released

Journal Article

Two NLR immune receptors acquired high-affinity binding to a fungal effector through convergent evolution of their integrated domain

MPS-Authors

There are no MPG-Authors in the publication available

External Resource

No external resources are shared

Fulltext (restricted access)

There are currently no full texts shared for your IP range.

Fulltext (public)

There are no public fulltexts stored in PuRe

Supplementary Material (public)

There is no public supplementary material available

Citation

Białas, A., Langner, T., Harant, A., Contreras, M., Stevenson, C., Lawson, D., et al. (2021). Two NLR immune receptors acquired high-affinity binding to a fungal effector through convergent evolution of their integrated domain. eLife, 10: e66961. doi:10.7554/eLife.66961.

Cite as: https://hdl.handle.net/21.11116/0000-000D-9009-6

Abstract

A subset of plant NLR immune receptors carry unconventional integrated domains in addition to their canonical domain architecture. One example is rice Pik-1 that comprises an integrated heavy metal-associated (HMA) domain. Here, we reconstructed the evolutionary history of Pik-1 and its NLR partner, Pik-2, and tested hypotheses about adaptive evolution of the HMA domain. Phylogenetic analyses revealed that the HMA domain integrated into Pik-1 before Oryzinae speciation over 15 million years ago and has been under diversifying selection. Ancestral sequence reconstruction coupled with functional studies showed that two Pik-1 allelic variants independently evolved from a weakly binding ancestral state to high-affinity binding of the blast fungus effector AVR-PikD. We conclude that for most of its evolutionary history the Pik-1 HMA domain did not sense AVR-PikD, and that different Pik-1 receptors have recently evolved through distinct biochemical paths to produce similar phenotypic outcomes. These findings highlight the dynamic nature of the evolutionary mechanisms underpinning NLR adaptation to plant pathogens.