Single-embryo RNA sequencing for continuous and sex-specific gene expression 
analysis on Drosophila

Pérez-Mojica, J Eduardo; Enders, Lennart; Lau, Kin H; Lempradl, Adelheid

doi:10.1016/j.xpro.2023.102535

Item

ITEM ACTIONSEXPORT

Add to Basket

Local TagsRelease HistoryDetailsSummary

Released

Journal Article

Single-embryo RNA sequencing for continuous and sex-specific gene expression analysis on Drosophila

MPS-Authors

Enders, Lennart
Department of Epigenetics, Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society;

External Resource

https://www.sciencedirect.com/science/article/pii/S2666166723005026?via%3Dihub
(Publisher version)

Fulltext (restricted access)

There are currently no full texts shared for your IP range.

Fulltext (public)

10.1016_j.it.2023.01.004.pdf
(Publisher version), 6MB

Supplementary Material (public)

There is no public supplementary material available

Citation

Pérez-Mojica, J. E., Enders, L., Lau, K. H., & Lempradl, A. (2023). Single-embryo RNA sequencing for continuous and sex-specific gene expression analysis on Drosophila. STAR Protocols, 4: 102535. doi:10.1016/j.xpro.2023.102535.

Cite as: https://hdl.handle.net/21.11116/0000-000D-B6F3-3

Abstract

Exploring early embryonic gene expression is challenging due to the rate of development and the limited material available. Here, we present a protocol for ordering Drosophila embryos along a developmental pseudo-time trajectory and determining the sex of the embryos using RNA-seq data. We describe steps for sample collection, RNA isolation, RNA-seq, and RNA-seq data processing. We then detail the establishment of a continuous transcriptome dataset for assessing gene expression throughout early development and in a sex-specific manner. For complete details on the use and execution of this protocol, please refer to Pérez-Mojica et al.¹.