A miniTurbo-based proximity labeling protocol to identify conditional protein 
interactomes in vivo in Myxococcus xanthus

Herfurth, Marco; Müller, Franziska; Søgaard-Andersen, Lotte; Glatter, Timo

doi:10.1016/j.xpro.2023.102657

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A miniTurbo-based proximity labeling protocol to identify conditional protein interactomes in vivo in Myxococcus xanthus

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Herfurth, Marco
Bacterial Adaption and Differentiation, Department of Ecophysiology, Max Planck Institute for Terrestrial Microbiology, Max Planck Society;

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Müller, Franziska
Bacterial Adaption and Differentiation, Department of Ecophysiology, Max Planck Institute for Terrestrial Microbiology, Max Planck Society;

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Søgaard-Andersen, Lotte
Bacterial Adaption and Differentiation, Department of Ecophysiology, Max Planck Institute for Terrestrial Microbiology, Max Planck Society;

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Glatter, Timo
Core Facility Mass Spectrometry and Proteomics, Max Planck Institute for Terrestrial Microbiology, Max Planck Society;

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https://doi.org/10.1016/j.xpro.2023.102657
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Citation

Herfurth, M., Müller, F., Søgaard-Andersen, L., & Glatter, T. (2023). A miniTurbo-based proximity labeling protocol to identify conditional protein interactomes in vivo in Myxococcus xanthus. STAR Protocols, 4(4): 102657. doi:10.1016/j.xpro.2023.102657.

Cite as: https://hdl.handle.net/21.11116/0000-000D-D87B-6

Abstract

Summary
Protein-protein interactions are foundational for many cellular processes. Such interactions are especially challenging to identify if they are transient or depend on environmental conditions. This protocol details steps to identify stable and transient protein interactomes in the bacterium Myxococcus xanthus using biotin ligase miniTurbo-based proximity labeling. We include instructions for optimizing the expression of control proteins, in vivo biotin labeling of bacteria grown on a surface or in suspension culture, enrichment of biotinylated proteins, and sample processing for proteomic analysis. For complete details on the use and execution of this protocol, please refer to Branon et al. (2018).1