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Journal Article

Histone methyltransferase activity of a Drosophila Polycomb group repressor complex

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Müller,  J       
Department Genetics, Max Planck Institute for Developmental Biology, Max Planck Society;

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Sengupta,  A
Department Genetics, Max Planck Institute for Developmental Biology, Max Planck Society;

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Citation

Müller, J., Hart, C., Francis, N., Vargas, M., Sengupta, A., Wild, B., et al. (2002). Histone methyltransferase activity of a Drosophila Polycomb group repressor complex. Cell, 111(2), 197-208. doi:0.1016/s0092-8674(02)00976-5.


Cite as: https://hdl.handle.net/21.11116/0000-000D-DC85-5
Abstract
Polycomb group (PcG) proteins maintain transcriptional repression during development, likely by creating repressive chromatin states. The Extra Sex Combs (ESC) and Enhancer of Zeste [E(Z)] proteins are partners in an essential PcG complex, but its full composition and biochemical activities are not known. A SET domain in E(Z) suggests this complex might methylate histones. We purified an ESC-E(Z) complex from Drosophila embryos and found four major subunits: ESC, E(Z), NURF-55, and the PcG repressor, SU(Z)12. A recombinant complex reconstituted from these four subunits methylates lysine-27 of histone H3. Mutations in the E(Z) SET domain disrupt methyltransferase activity in vitro and HOX gene repression in vivo. These results identify E(Z) as a PcG protein with enzymatic activity and implicate histone methylation in PcG-mediated silencing.