English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT

Released

Talk

A neural-crest-derived glial lineage in the zebrafish: Coupling in vivo imaging and genetic analysis

MPS-Authors
/persons/resource/persons285532

Gilmour,  DT       
Department Genetics, Max Planck Institute for Developmental Biology, Max Planck Society;

/persons/resource/persons224247

Maischein,  HM
Department Genetics, Max Planck Institute for Developmental Biology, Max Planck Society;

/persons/resource/persons271460

Nüsslein-Volhard,  C       
Department Genetics, Max Planck Institute for Developmental Biology, Max Planck Society;

External Resource
No external resources are shared
Fulltext (restricted access)
There are currently no full texts shared for your IP range.
Fulltext (public)
There are no public fulltexts stored in PuRe
Supplementary Material (public)
There is no public supplementary material available
Citation

Gilmour, D., Maischein, H., & Nüsslein-Volhard, C. (2001). A neural-crest-derived glial lineage in the zebrafish: Coupling in vivo imaging and genetic analysis. Talk presented at Sixtieth Annual Meeting of the Society for Developmental Biology (SDB 2001). Seattle, WA, USA. 2001-07-18 - 2001-07-22.


Cite as: https://hdl.handle.net/21.11116/0000-000D-DCF5-7
Abstract
Neural crest cells (NCCs) are a population of pluripotent cells that migrate from their birthplace at the dorsal neural tube to a wide number of destinations in the developing embryo. The NCCs that become the glia of the PNS, unlike other crest, colocalize with embryonic structures that are also quickly moving during develop- ment, namely, the axon bundles they ensheath. It is however unclear whether the axons determine the final positions of the glia or vice versa. By making transgenic zebrafish lines expressing GFP under the control of the zFoxD3 promoter we have been able to follow the migration of one subset of NC-derived glia in living embryos. These glia migrate as chains with the leading cells acting as “pioneers” during pathfinding. DiI labeling of their associated axons shows that glia migration is tightly coupled to axon exten- sion, with the pioneer glial cell apparently “riding” on the axon growth cone itself. We show that these GFP-labeled glia always colocalize with extending axons even in mutants that strongly affect axon pathfinding, showing that the axons provide instructive guidance cues necessary for the migration of this NCC lineage. We have isolated a group of mutants showing defects in neural crest morphogenesis and we are currently investigating these pheno- types in living mutant embryos expressing the zFoxD3 transgene. This approach has allowed us to identify a mutant in which NCC-derived glia are no longer able to respond to axon-derived guidance cues.