Lysis induction of Escherichia coli by the cloned lysis protein of the phage 
MS2 depends on the presence of osmoregulatory membrane-derived oligosaccharides

Höltje, J-V; Fiedler, W; Rotering, H; Walderich, B; Van Duin, J

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Lysis induction of Escherichia coli by the cloned lysis protein of the phage MS2 depends on the presence of osmoregulatory membrane-derived oligosaccharides

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Höltje, J-V
Department Biochemistry, Max Planck Institute for Developmental Biology, Max Planck Society;

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Walderich, B
Department Biochemistry, Max Planck Institute for Developmental Biology, Max Planck Society;

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Höltje, J.-V., Fiedler, W., Rotering, H., Walderich, B., & Van Duin, J. (1988). Lysis induction of Escherichia coli by the cloned lysis protein of the phage MS2 depends on the presence of osmoregulatory membrane-derived oligosaccharides. The Journal of Biological Chemistry, 263(8), 3539-3541.

Cite as: https://hdl.handle.net/21.11116/0000-000D-EA89-1

Abstract

Expression of the cloned lysis protein of phage MS2, which is sufficient to lyse wild type Escherichia coli, does not cause lysis of mutants lacking the osmoregulatory membrane-derived oligosaccharides (MDO). The lysis gene product normally found in the membrane fraction was not stably inserted into the membranes of a mdoA mutant; rather degradation and release from the membrane occurred. Gentle plasmolysis of the MDO-lacking mutant clearly showed an increased periplasmic space as compared to wild type cells. It is concluded that the MDOs play an important role in maintaining a proper arrangement of inner and outer membrane, a prerequisite for a functional insertion of the MS2 lysis protein.