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Journal Article

One-step purification procedure for UDP-N-acetylmuramyl-peptide murein precursors from Bacillus cereus

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Kohlrausch,  U
Department Biochemistry, Max Planck Institute for Developmental Biology, Max Planck Society;

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Höltje,  J-V
Department Biochemistry, Max Planck Institute for Developmental Biology, Max Planck Society;

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Citation

Kohlrausch, U., & Höltje, J.-V. (1991). One-step purification procedure for UDP-N-acetylmuramyl-peptide murein precursors from Bacillus cereus. FEMS Microbiology Letters, 62(2-3), 253-257. doi:10.1016/0378-1097(91)90166-8.


Cite as: https://hdl.handle.net/21.11116/0000-000D-EAA1-5
Abstract
A method is described for the rapid isolation of the activated murein precursors UDP-N-acetyl-muramyl-pentapeptide (UDP-MurNAc-pentapeptide) and UDP-MurNAc-tripeptide from Bacillus cereus. After accumulation of the precursors by inhibition of murein synthesis either in the presence of vancomycin (for the pentapeptide precursor) or D-cycloserine (for the tripeptide precursor) the cells were extracted with boiling water. Prior to high pressure liquid chromatography the material was freed from acid precipitable material. UDP-MurNAc-penta- and tripeptide were separated from other components by reversed-phase HPLC on Hypersil ODS using isocratic elution conditions with sodium phosphate buffer. The precursors were obtained with at least 98% purity and a yield of about 50 mumol from a 10-l culture of B. cereus.