English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT

Released

Journal Article

In situ tracking of exoenzyme activity using droplet luminescence concentrators for ratiometric detection of bacteria

MPS-Authors
/persons/resource/persons273714

Baryzewska,  Agata
Lukas Zeininger, Kolloidchemie, Max Planck Institute of Colloids and Interfaces, Max Planck Society;

/persons/resource/persons213998

Roth,  Christian
Christian Roth, Biomolekulare Systeme, Max Planck Institute of Colloids and Interfaces, Max Planck Society;

/persons/resource/persons121849

Seeberger,  Peter H.       
Peter H. Seeberger - Vaccine Development, Biomolekulare Systeme, Max Planck Institute of Colloids and Interfaces, Max Planck Society;

/persons/resource/persons243119

Zeininger,  Lukas       
Lukas Zeininger, Kolloidchemie, Max Planck Institute of Colloids and Interfaces, Max Planck Society;

External Resource
No external resources are shared
Fulltext (restricted access)
There are currently no full texts shared for your IP range.
Fulltext (public)

Article.pdf
(Publisher version), 3MB

Supplementary Material (public)
There is no public supplementary material available
Citation

Baryzewska, A., Roth, C., Seeberger, P. H., & Zeininger, L. (2023). In situ tracking of exoenzyme activity using droplet luminescence concentrators for ratiometric detection of bacteria. ACS Sensors, 8(11), 4143-4151. doi:10.1021/acssensors.3c01385.


Cite as: https://hdl.handle.net/21.11116/0000-000D-ED02-6
Abstract
We demonstrate a novel, rapid, and cost-effective biosensing paradigm that is based on an in situ visualization of bacterial exoenzyme activity using biphasic Janus emulsion droplets. Sensitization of the droplets toward dominant extracellular enzymes of bacterial pathogens is realized via selective functionalization of one hemisphere of Janus droplets with enzyme-cleavable surfactants. Surfactant cleavage results in an interfacial tension increase at the respective droplet interface, which readily transduces into a microscopically detectable change of the internal droplet morphologies. A macroscopic fluorescence read-out of such morphological transitions is obtained via ratiometrically recording the angle-dependent anisotropic emission signatures of perylene-containing droplets from two different angles. The optical read-out method facilitates detection of marginal morphological responses of polydisperse droplet samples that can be easily produced in any environment. The performance of Janus droplets as powerful optical transducers and signal amplifiers is highlighted by rapid (≺4 h) and cost-effective antibody and DNA-free identification of three major foodborne pathogens, with detection thresholds of below 10 CFU mL–1 for Salmonella and ≺102 to 103 CFU mL–1 for Listeria and Escherichia coli.