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Microtubule-dependent recruitment of Staufen-green fluorescent protein into large RNA-containing granules and subsequent dendritic transport in living hippocampal neurons

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Köhrmann,  M       
Research Group Molecular Events at the Mammalian Synapse, Max Planck Institute for Developmental Biology, Max Planck Society;
Department Physical Biology, Max Planck Institute for Developmental Biology, Max Planck Society;

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Kiebler,  MA       
Research Group Molecular Events at the Mammalian Synapse, Max Planck Institute for Developmental Biology, Max Planck Society;
Department Physical Biology, Max Planck Institute for Developmental Biology, Max Planck Society;

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Citation

Köhrmann, M., Luo, M., Kaether, C., DesGroseillers, L., Dotti, C., & Kiebler, M. (1999). Microtubule-dependent recruitment of Staufen-green fluorescent protein into large RNA-containing granules and subsequent dendritic transport in living hippocampal neurons. Molecular Biology of the Cell, 10(9), 2945-2953. doi:10.1091/mbc.10.9.2945.


Cite as: https://hdl.handle.net/21.11116/0000-000D-F5E0-1
Abstract
Dendritic mRNA transport and local translation at individual potentiated synapses may represent an elegant way to form synaptic memory. Recently, we characterized Staufen, a double-stranded RNA-binding protein, in rat hippocampal neurons and showed its presence in large RNA-containing granules, which colocalize with microtubules in dendrites. In this paper, we transiently transfect hippocampal neurons with human Staufen-green fluorescent protein (GFP) and find fluorescent granules in the somatodendritic domain of these cells. Human Stau-GFP granules show the same cellular distribution and size and also contain RNA, as already shown for the endogenous Stau particles. In time-lapse videomicroscopy, we show the bidirectional movement of these Staufen-GFP-labeled granules from the cell body into dendrites and vice versa. The average speed of these particles was 6.4 microm/min with a maximum velocity of 24. 3 microm/min. Moreover, we demonstrate that the observed assembly into granules and their subsequent dendritic movement is microtubule dependent. Taken together, we have characterized a novel, nonvesicular, microtubule-dependent transport pathway involving RNA-containing granules with Staufen as a core component. This is the first demonstration in living neurons of movement of an essential protein constituent of the mRNA transport machinery.