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Book Chapter

The intermediate filament systems in the eye lens


Dahm,  R       
Department Genetics, Max Planck Institute for Developmental Biology, Max Planck Society;

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Perng, M., Sandilands, A., Kuszak, J., Dahm, R., Wegener, A., Prescott, A., et al. (2004). The intermediate filament systems in the eye lens. In B. Omary, & P. Coulombe (Eds.), Intermediate Filament Cytoskeleton (pp. 597-624). Amsterdam, The Netherlands: Elsevier Academic Press.

Cite as: https://hdl.handle.net/21.11116/0000-000D-F99B-C
This chapter describes the basic methods to purify important lens fractions and to track the distribution of IF proteins in the eye lens and their associated proteins. It also provides the description of the methods used for the preparation, sectioning, and examination of eye lenses. The IF proteins of the lens help in determining its optical properties. Whereas, a transparent lens is normal, a lens with either a reduced level of transparency or a localized opacity is abnormal and/or pathological. One of the pitfalls in the purification of lens proteins is proteolysis, especially when urea buffers are used, but the methods in the chapter describe the effective purification of CP49, filensin, and vimentin for use in vitro assembly and structural studies. The animal models can help to understand the mechanisms of cataractogensis, and in combination with the optical studies the futuristic possibility of regenerating superlenses for combating cataract may be expected.