Vectors for efficient and high-throughput construction of fluorescent 
drosophila reporters using the PhiC31 site-specific integration system

Boy, AL; Zhai, Z; Habring-Müller, A; Kussler-Schneider, Y; Kaspar, P; Lohmann, I

doi:10.1002/dvg.20637

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Vectors for efficient and high-throughput construction of fluorescent drosophila reporters using the PhiC31 site-specific integration system

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Habring-Müller, A
Department Molecular Biology, Max Planck Institute for Developmental Biology, Max Planck Society;

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Citation

Boy, A., Zhai, Z., Habring-Müller, A., Kussler-Schneider, Y., Kaspar, P., & Lohmann, I. (2010). Vectors for efficient and high-throughput construction of fluorescent drosophila reporters using the PhiC31 site-specific integration system. Genesis, 48(7), 452-456. doi:10.1002/dvg.20637.

Cite as: https://hdl.handle.net/21.11116/0000-000E-073E-6

Abstract

The fruit fly Drosophila is a leading model system for the study of transcriptional control by cis-regulatory elements or enhancers. Here, we present a rapid and highly efficient system for the large-scale analysis of enhancer elements, site-specifically integrated into the Drosophila genome. This system, which is scalable for either small projects or high-throughput approaches, makes use of the Gateway cloning technology and the PhiC31 site-specific integration system, which allows the insertion of constructs at predetermined genomic locations. Thus, this system allows not only a fast and easy analysis of reporter gene expression in live animals, but also the simultaneous analysis of different regulatory outputs on a cellular resolution by recombining in the same animal distinct enhancer elements fused to different fluorescent proteins.