English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT

Released

Journal Article

Dominance of Sulfuritalea species in nitrate-depleted water of a stratified freshwater lake and arsenate respiration ability within the genus

MPS-Authors

Watanabe,  Tomohiro
external;
Max Planck Institute for Terrestrial Microbiology_others, Max Planck Institute for Terrestrial Microbiology, Max Planck Society;

External Resource
Fulltext (restricted access)
There are currently no full texts shared for your IP range.
Fulltext (public)
There are no public fulltexts stored in PuRe
Supplementary Material (public)
There is no public supplementary material available
Citation

Watanabe, T., Miura, A., Iwata, T., Kojima, H., & Fukui, M. (2017). Dominance of Sulfuritalea species in nitrate-depleted water of a stratified freshwater lake and arsenate respiration ability within the genus. ENVIRONMENTAL MICROBIOLOGY REPORTS, 9(5), 522-527. doi:10.1111/1758-2229.12557.


Cite as: https://hdl.handle.net/21.11116/0000-000E-0EF7-D
Abstract
Facultative autotrophs of the genus Sulfuritalea within the class
Betaproteobacteria have been predicted to be an important bacterial
population in stratified freshwater lakes based on previous PCR-based
studies. Here, we designed a new probe specific for the genus
Sulfuritalea and performed catalysed reporter deposition-fluorescence in
situ hybridisation to enumerate cells of Sulfuritalea species throughout
the water column in a stratified freshwater lake. The cells stained with
the Sulfuritalea-specific probe were detected in all hypoxic water
samples collected in different seasons and years. Their abundance ranged
from 1.4 x 10(4) to 2.1 x 10(5) cells ml(-1), corresponding to 0.5-5.5%
of the total DAPI-stained cells and 2.3-15% of the total bacterial
cells. A high abundance of Sulfuritalea species was recorded in hypoxic
water samples without nitrate, which is the only known anaerobic
electron acceptor for Sulfuritalea. Nitrate-independent anaerobic
respiration was further investigated using a single cultured
representative of this genus, and its growth via arsenate respiration
was experimentally demonstrated. In conclusion, Sulfuritalea species
were found to be a major component of the planktonic bacterial community
in nitrate-depleted hypoxic water, where arsenate respiration is one of
the possible energy metabolisms of Sulfuritalea.