Arp2/3 complex and the pentose phosphate pathway regulate late phases of 
neutrophil swarming

Glaser, Katharina M; Doon-Ralls, Jacob; Walters, Nicole; Rima, Xilal Y; Rambold, Angelika; Réategui, Eduardo; Lämmermann, Tim

doi:10.1016/j.isci.2023.108656

Item

ITEM ACTIONSEXPORT

Add to Basket

Local TagsRelease HistoryDetailsSummary

Released

Journal Article

Arp2/3 complex and the pentose phosphate pathway regulate late phases of neutrophil swarming

MPS-Authors

Glaser, Katharina M
Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society;

/persons/resource/persons241710

Rambold, Angelika
Department of Developmental Immunobiology, Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society;

/persons/resource/persons204285

Lämmermann, Tim
Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society;

External Resource

https://www.sciencedirect.com/science/article/pii/S2589004223027335?via%3Dihub
(Publisher version)

Fulltext (restricted access)

There are currently no full texts shared for your IP range.

Fulltext (public)

10.1016_j.isci.2023.108656.pdf
(Publisher version), 11MB

Supplementary Material (public)

There is no public supplementary material available

Citation

Glaser, K. M., Doon-Ralls, J., Walters, N., Rima, X. Y., Rambold, A., Réategui, E., et al. (2024). Arp2/3 complex and the pentose phosphate pathway regulate late phases of neutrophil swarming. iScience, 27: 108656. doi:10.1016/j.isci.2023.108656.

Cite as: https://hdl.handle.net/21.11116/0000-000E-3932-A

Abstract

Neutrophil swarming is an essential process of the neutrophil response to many pathological conditions. Resultant neutrophil accumulations are hallmarks of acute tissue inflammation and infection, but little is known about their dynamic regulation. Technical limitations to spatiotemporally resolve individual cells in dense neutrophil clusters and manipulate these clusters in situ have hampered recent progress. We here adapted an in vitro swarming-on-a-chip platform for the use with confocal laser-scanning microscopy to unravel the complexity of single-cell responses during neutrophil crowding. Confocal sectioning allowed the live visualization of subcellular components, including mitochondria, cell membranes, cortical actin, and phagocytic cups, inside neutrophil clusters. Based on this experimental setup, we identify that chemical inhibition of the Arp2/3 complex causes cell death in crowding neutrophils. By visualizing spatiotemporal patterns of reactive oxygen species (ROS) production in developing neutrophil swarms, we further demonstrate a regulatory role of the metabolic pentose phosphate pathway for ROS production and neutrophil cluster growth.