A new method to prepare sections from amphibian embryos for immunohistology

Hausen, P

doi:10.1515/znc-1988-9-1021

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A new method to prepare sections from amphibian embryos for immunohistology

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Hausen, P
Department Cell Biology, Max Planck Institute for Developmental Biology, Max Planck Society;

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Hausen, P. (1988). A new method to prepare sections from amphibian embryos for immunohistology. Zeitschrift für Naturforschung, C: Journal of Biosciences, 43(9-10), 765-768. doi:10.1515/znc-1988-9-1021.

Cite as: https://hdl.handle.net/21.11116/0000-000E-4650-9

Abstract

The method involves impregnation of fixed embryos with a solution of plexiglass. The solvent is evaporated slowly. Blocks of plexiglass containing the specimen are thus formed. Sections (1-2 microns) are stretched and attached by an unconvential method. Immunohistological techniques allow antigen localization in a combination of high resolution, excellent quality of the sections and low background.