Single RNA polymerase binding site isolated

Heyden, B; Nüsslein, C; Schaller, H

doi:10.1038/newbio240009a0

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Single RNA polymerase binding site isolated

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Heyden, B., Nüsslein, C., & Schaller, H. (1972). Single RNA polymerase binding site isolated. Nature New Biology, 240(96), 9-12. doi:10.1038/newbio240009a0.

Cite as: https://hdl.handle.net/21.11116/0000-000E-5754-2

Abstract

One approach to study the structure of promoter regions1–3 is to isolate RNA polymerase binding sites. Several attempts have been made to isolate such sites as protected DNA fragments4–10, but so far the DNA isolated has not definitely shown to be only one or a few specific sites. We report here the isolation of a single RNA polymerase binding site from the replicative form (RF) DNA of bacteriophage fd. The site as isolated is a short double-stranded DNA with a unique nucleotide sequence.