English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT

Released

Journal Article

A new hyperelastic lookup table for RT-DC

MPS-Authors

Wittwer,  Lukas Daniel
Max-Planck-Zentrum für Physik und Medizin, Max Planck Institute for the Science of Light, Max Planck Society;
Guck Division, Max Planck Institute for the Science of Light, Max Planck Society;

/persons/resource/persons255636

Reichel,  Felix
Max-Planck-Zentrum für Physik und Medizin, Max Planck Institute for the Science of Light, Max Planck Society;
Guck Division, Max Planck Institute for the Science of Light, Max Planck Society;

/persons/resource/persons248158

Mueller,  Paul
Max-Planck-Zentrum für Physik und Medizin, Max Planck Institute for the Science of Light, Max Planck Society;
Guck Division, Max Planck Institute for the Science of Light, Max Planck Society;

/persons/resource/persons241284

Guck,  Jochen
Max-Planck-Zentrum für Physik und Medizin, Max Planck Institute for the Science of Light, Max Planck Society;
Guck Division, Max Planck Institute for the Science of Light, Max Planck Society;
Dept. of Physics, Friedrich-Alexander-Universität Erlangen-Nürnberg (FAU), Erlangen, Germany;

External Resource
No external resources are shared
Fulltext (restricted access)
There are currently no full texts shared for your IP range.
Fulltext (public)

Soft Matter 2023 Wittwer.pdf
(Publisher version), 4MB

Supplementary Material (public)
There is no public supplementary material available
Citation

Wittwer, L. D., Reichel, F., Mueller, P., Guck, J., & Aland, S. (2023). A new hyperelastic lookup table for RT-DC. Soft Matter, 19(11), 2064-2073. doi:10.1039/d2sm01418a.


Cite as: https://hdl.handle.net/21.11116/0000-000E-7234-7
Abstract
Real-time deformability cytometry (RT-DC) is an established method that quantifies features like size, shape, and stiffness for whole cell populations on a single-cell level in real-time. A lookup table (LUT) disentangles the experimentally derived steady-state cell deformation and the projected area to extract the cell stiffness in the form of the Young's modulus. So far, two lookup tables exist but are limited to simple linear material models and cylindrical channel geometries. Here, we present two new lookup tables for RT-DC based on a neo-Hookean hyperelastic material numerically derived by simulations based on the finite element method in square and cylindrical channel geometries. At the same time, we quantify the influence of the shear-thinning behavior of the surrounding medium on the stationary deformation of cells in RT-DC and discuss the applicability and impact of the proposed LUTs regarding past and future RT-DC data analysis. Additionally, we provide insights about the cell strain and stresses, as well as the influence resulting from the rotational symmetric assumption on the cell deformation and volume estimation. The new lookup tables and the numerical cell shapes are made freely available.