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A new hyperelastic lookup table for RT-DC

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Wittwer,  Lukas Daniel
Guck Division, Max-Planck-Zentrum für Physik und Medizin, Max Planck Institute for the Science of Light, Max Planck Society;
Guck Division, Max Planck Institute for the Science of Light, Max Planck Society;

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Reichel,  Felix
Guck Division, Max-Planck-Zentrum für Physik und Medizin, Max Planck Institute for the Science of Light, Max Planck Society;
Guck Division, Max Planck Institute for the Science of Light, Max Planck Society;

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Mueller,  Paul
Guck Division, Max-Planck-Zentrum für Physik und Medizin, Max Planck Institute for the Science of Light, Max Planck Society;
Guck Division, Max Planck Institute for the Science of Light, Max Planck Society;

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Guck,  Jochen
Guck Division, Max-Planck-Zentrum für Physik und Medizin, Max Planck Institute for the Science of Light, Max Planck Society;
Guck Division, Max Planck Institute for the Science of Light, Max Planck Society;
Friedrich-Alexander-Universität Erlangen-Nürnberg, External Organizations;

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Soft Matter 2023 Wittwer.pdf
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Citation

Wittwer, L. D., Reichel, F., Mueller, P., Guck, J., & Aland, S. (2023). A new hyperelastic lookup table for RT-DC. Soft Matter, 19(11), 2064-2073. doi:10.1039/d2sm01418a.


Cite as: https://hdl.handle.net/21.11116/0000-000E-7234-7
Abstract
Real-time deformability cytometry (RT-DC) is an established method that quantifies features like size, shape, and stiffness for whole cell populations on a single-cell level in real-time. A lookup table (LUT) disentangles the experimentally derived steady-state cell deformation and the projected area to extract the cell stiffness in the form of the Young's modulus. So far, two lookup tables exist but are limited to simple linear material models and cylindrical channel geometries. Here, we present two new lookup tables for RT-DC based on a neo-Hookean hyperelastic material numerically derived by simulations based on the finite element method in square and cylindrical channel geometries. At the same time, we quantify the influence of the shear-thinning behavior of the surrounding medium on the stationary deformation of cells in RT-DC and discuss the applicability and impact of the proposed LUTs regarding past and future RT-DC data analysis. Additionally, we provide insights about the cell strain and stresses, as well as the influence resulting from the rotational symmetric assumption on the cell deformation and volume estimation. The new lookup tables and the numerical cell shapes are made freely available.