Novel secreted effectors conserved among smut fungi contribute to the virulence 
of Ustilago maydis

Schuster, Mariana; Schweizer, Gabriel; Reissmann, Stefanie; Happel, Petra; Assmann, Daniela; Roessel, Nicole; Gueldener, Ulrich; Mannhaupt, Gertrud; Ludwig, Nicole; Winterberg, Sarah; Pellegrin, Clément; Tanaka, Shigeyuki; Vincon, Volker; Lo Presti, Libera; Wang, Lei; Bender, Lena; Gonzalez, Carla; Vranes, Miroslav; Kaemper, Joerg; Seong, Kyungyong; Krasileva, Ksenia; Kahmann, Regine

doi:10.1094/MPMI-09-23-0139-FI

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Novel secreted effectors conserved among smut fungi contribute to the virulence of Ustilago maydis

MPS-Authors

/persons/resource/persons254702

Schuster, Mariana
Department of Organismic Interactions, Alumni, Max Planck Institute for Terrestrial Microbiology, Max Planck Society;

/persons/resource/persons254707

Schweizer, Gabriel
Department of Organismic Interactions, Alumni, Max Planck Institute for Terrestrial Microbiology, Max Planck Society;

/persons/resource/persons254635

Reissmann, Stefanie
Department of Organismic Interactions, Alumni, Max Planck Institute for Terrestrial Microbiology, Max Planck Society;

/persons/resource/persons254333

Happel, Petra
Department of Organismic Interactions, Alumni, Max Planck Institute for Terrestrial Microbiology, Max Planck Society;

/persons/resource/persons254107

Assmann, Daniela
Department of Organismic Interactions, Alumni, Max Planck Institute for Terrestrial Microbiology, Max Planck Society;

/persons/resource/persons254650

Roessel, Nicole
Department of Organismic Interactions, Alumni, Max Planck Institute for Terrestrial Microbiology, Max Planck Society;

/persons/resource/persons254520

Mannhaupt, Gertrud
Department of Organismic Interactions, Alumni, Max Planck Institute for Terrestrial Microbiology, Max Planck Society;

/persons/resource/persons254508

Ludwig, Nicole
Department of Organismic Interactions, Alumni, Max Planck Institute for Terrestrial Microbiology, Max Planck Society;

/persons/resource/persons254848

Winterberg, Sarah
Department of Organismic Interactions, Alumni, Max Planck Institute for Terrestrial Microbiology, Max Planck Society;

/persons/resource/persons263990

Pellegrin, Clément
Department of Organismic Interactions, Alumni, Max Planck Institute for Terrestrial Microbiology, Max Planck Society;

/persons/resource/persons254754

Tanaka, Shigeyuki
Department of Organismic Interactions, Alumni, Max Planck Institute for Terrestrial Microbiology, Max Planck Society;

/persons/resource/persons254795

Vincon, Volker
Department of Organismic Interactions, Alumni, Max Planck Institute for Terrestrial Microbiology, Max Planck Society;

/persons/resource/persons254504

Lo Presti, Libera
Department of Organismic Interactions, Alumni, Max Planck Institute for Terrestrial Microbiology, Max Planck Society;

/persons/resource/persons254820

Wang, Lei
Department of Organismic Interactions, Alumni, Max Planck Institute for Terrestrial Microbiology, Max Planck Society;

Bender, Lena
Department of Organismic Interactions, Alumni, Max Planck Institute for Terrestrial Microbiology, Max Planck Society;

/persons/resource/persons267053

Gonzalez, Carla
Emeriti Molecular Phytopathology, Max Planck Institute for Terrestrial Microbiology, Max Planck Society;

/persons/resource/persons254413

Kahmann, Regine
Emeriti Molecular Phytopathology, Max Planck Institute for Terrestrial Microbiology, Max Planck Society;

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Citation

Schuster, M., Schweizer, G., Reissmann, S., Happel, P., Assmann, D., Roessel, N., et al. (2024). Novel secreted effectors conserved among smut fungi contribute to the virulence of Ustilago maydis. Molecular Plant-Microbe Interactions. doi:10.1094/MPMI-09-23-0139-FI.

Cite as: https://hdl.handle.net/21.11116/0000-000E-7FEA-D

Abstract

Fungal pathogens deploy a set of molecules (proteins, specialized metabolites, and sRNA), so called effectors, to aid the infection process. In comparison to other plant pathogens, smut fungi have small genomes and secretomes of 20 Mb and around 500 proteins, respectively.. Previous comparative genomic studies have shown that many secreted effector proteins without known domains i.e., novel, are conserved only in the Ustilaginaceae family. By analyzing the secretome of 11 species within Ustilaginaceae, we identified 53 core homologous groups commonly present in this lineage. By collecting existing mutants and generating additional ones, we gathered 44 Ustilago maydis strains lacking single core effectors as well as 9 strains contain-ing multiple deletions of core effector gene families. Pathogenicity assays revealed that 20 of these 53 mutant strains were affected in virulence. Among the 33 mutants that had no obvious phenotypic changes, 13 carried additional, sequence-divergent, structurally similar paralogs. We report a virulence contribution of seven previously uncharacterized single core effectors and of one effector family. Our results help to prioritize effectors for understanding U. maydis virulence and provide genetic re-sources for further characterization.