Datensatz

Zusammenfassung

To understand the evolution of developmental process at the microevolutionary level, it is important to study the biogeography and the evolutionary alterations of the developmental processes in detail. To acuminate this, we are working on the satellite model Pristionchus pacificus. The genus Pristionchus has a worldwide distribution and different strains of Pristionchus pacificus exist from Washington (PS 1843), Hawaii (JU 138), Ontario (AF 8130), Poland (RS 106) and the wild type strain from California (PS 312). Previous AFLP studies showed a high degree of polymorphisms between the strains from Washington, Hawaii and California. BAC end sequencing and SSCP analysis of BAC end fragments between the strains from California and Washington confirmed these original observations. To obtain a better picture of the distribution of polymorphisms between the strains of P. pacificus, we have chosen a random 5 Kb region on Chromosome III that contains no open reading frame. Our results showed a high degree of polymorphism (3.46%) in both Washington and Hawaii when compared to the laboratory strain California. There were ~ 0.7% transitions, ~ 0.5% transversions, ~ 0.3% insertions and ~ 0.1% deletions. Most of the polymorphisms observed were single base pair events except a few which were up to 5 bp . Further studies are underway to extend this study, comparing the ceh-20 and ceh-40 genes in these strains and to eventually include the different species’ of Pristionchus namely P. maupasi and P. lheritieri.