Improving the UV detection sensitivity of condensed polyacrylamide gel-filled 
capillaries using non-flow buffer-filled capillaries as a detection cell

Chen, Y; Höltje, J-V; Schwarz, U

doi:10.1016/0021-9673(94)01137-4

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Improving the UV detection sensitivity of condensed polyacrylamide gel-filled capillaries using non-flow buffer-filled capillaries as a detection cell

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Chen, Y
Department Biochemistry, Max Planck Institute for Developmental Biology, Max Planck Society;

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Höltje, J-V
Department Biochemistry, Max Planck Institute for Developmental Biology, Max Planck Society;

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Schwarz, U
Department Biochemistry, Max Planck Institute for Developmental Biology, Max Planck Society;

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Citation

Chen, Y., Höltje, J.-V., & Schwarz, U. (1995). Improving the UV detection sensitivity of condensed polyacrylamide gel-filled capillaries using non-flow buffer-filled capillaries as a detection cell. Journal of Chromatography A, 700(1-2), 35-42. doi:10.1016/0021-9673(94)01137-4.

Cite as: https://hdl.handle.net/21.11116/0000-000E-A4EE-D

Abstract

Capillary coupling techniques are suggested for the connection of a non-flow buffer-filled capillary to gel-filled capillaries as a low-background detection cell. The UV detection sensitivity of the resulting capillaries is improved at least fivefold, as demonstrated by using polyamino acids as test samples. The detection sensitivity can be further improved by using a low-background running buffer. Several ways are suggested to overcome the problems of resolution decrease and baseline drif which were observed after coupling.