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Core PCP mutations affect short-time mechanical properties but not tissue morphogenesis in the Drosophila pupal wing.

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Piscitello-Gómez,  Romina
Max Planck Institute for Molecular Cell Biology and Genetics, Max Planck Society;

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Gruber,  Franz
Max Planck Institute for Molecular Cell Biology and Genetics, Max Planck Society;

/cone/persons/resource/persons231555

Krishna,  Abhijeet
Max Planck Institute for Molecular Cell Biology and Genetics, Max Planck Society;

/cone/persons/resource/persons219459

Modes,  Carl D.
Max Planck Institute for Molecular Cell Biology and Genetics, Max Planck Society;

/cone/persons/resource/persons145744

Jülicher,  Frank
Max Planck Institute for Molecular Cell Biology and Genetics, Max Planck Society;

/cone/persons/resource/persons219124

Dye,  Natalie
Max Planck Institute for Molecular Cell Biology and Genetics, Max Planck Society;

/cone/persons/resource/persons219126

Eaton,  Suzanne
Max Planck Institute for Molecular Cell Biology and Genetics, Max Planck Society;

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Citation

Piscitello-Gómez, R., Gruber, F., Krishna, A., Duclut, C., Modes, C. D., Popović, M., et al. (2023). Core PCP mutations affect short-time mechanical properties but not tissue morphogenesis in the Drosophila pupal wing. eLife, 12: e85581. doi:10.7554/eLife.85581.


Cite as: https://hdl.handle.net/21.11116/0000-000E-AAC4-5
Abstract
How morphogenetic movements are robustly coordinated in space and time is a fundamental open question in biology. We study this question using the wing of Drosophila melanogaster, an epithelial tissue that undergoes large-scale tissue flows during pupal stages. Previously, we showed that pupal wing morphogenesis involves both cellular behaviors that allow relaxation of mechanical tissue stress, as well as cellular behaviors that appear to be actively patterned (Etournay et al., 2015). Here, we show that these active cellular behaviors are not guided by the core planar cell polarity (PCP) pathway, a conserved signaling system that guides tissue development in many other contexts. We find no significant phenotype on the cellular dynamics underlying pupal morphogenesis in mutants of core PCP. Furthermore, using laser ablation experiments, coupled with a rheological model to describe the dynamics of the response to laser ablation, we conclude that while core PCP mutations affect the fast timescale response to laser ablation they do not significantly affect overall tissue mechanics. In conclusion, our work shows that cellular dynamics and tissue shape changes during Drosophila pupal wing morphogenesis do not require core PCP as an orientational guiding cue.