MINSTED tracking of single biomolecules

Scheiderer, Lukas; von der Emde, Henrik; Hesselink, Mira; Weber, Michael; Hell, Stefan W.

doi:10.1038/s41592-024-02209-6

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学術論文

MINSTED tracking of single biomolecules

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Scheiderer, Lukas
Optical Nanoscopy, Max Planck Institute for Medical Research, Max Planck Society;

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Hell, Stefan W.
Optical Nanoscopy, Max Planck Institute for Medical Research, Max Planck Society;

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https://www.nature.com/articles/s41592-024-02209-6
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引用

Scheiderer, L., von der Emde, H., Hesselink, M., Weber, M., & Hell, S. W. (2024). MINSTED tracking of single biomolecules. Nature Methods, 1-9. doi:10.1038/s41592-024-02209-6.

引用: https://hdl.handle.net/21.11116/0000-000E-B32E-5

要旨

Here we show that MINSTED localization, a method whereby the position of a fluorophore is identified with precisely controlled beams of a STED microscope, tracks fluorophores and hence labeled biomolecules with nanometer/millisecond spatiotemporal precision. By updating the position for each detected photon, MINSTED recognizes fluorophore steps of 16 nm within <250 μs using about 13 photons. The power of MINSTED tracking is demonstrated by resolving the stepping of the motor protein kinesin-1 walking on microtubules and switching protofilaments.